We narrowed to 6,879 results for: crispr cas9 plasmids
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Plasmid#179334PurposeNT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).DepositorInsertPtac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRMutationCas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-US-ECasE
Plasmid#83961PurposePiggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.DepositorInsertERT2-spCas9-ERT2
ExpressionMammalianMutationplease see depositor comments belowPromoterU6 (gRNA), EF1a (ERT2-Cas9-ERT2)Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseExpressionBacterialPromoternative cas9 promoterAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalpha
Plasmid#160229Purposeplaces LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal platesDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x2
Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCM4.4
Plasmid#217648Purposean ermE*p-cas9-driven expression, as an alternative plasmidDepositorInsertCodon Optimized SpCas9
UseCRISPRAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCP-tRNA
Plasmid#133812PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosisDepositorInsertcassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
UseCRISPRAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT-tRNA
Plasmid#133813PurposeCRISPR-Cas9 system for genetic manipulation of Candida tropicalisDepositorInsertcassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
UseCRISPRAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGMC00018 (aka pRC0082)
Plasmid#195320PurposeMinimal plasmid backbone with multiple cloning site for HDR donor DNA constructionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterial and MammalianAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Afp
Plasmid#99697PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAVDepositorAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti SpBsmBI sgRNA Hygro
Plasmid#62205PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6Available SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJAT74
Plasmid#204296PurposepJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330 Mouse 5' Npm1 gRNA
Plasmid#127900PurposeWT Cas9 Vector targeting the 5' end of the mouse Npm1 geneDepositorInsertgRNA/Cas9
UseCRISPRAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 Mouse 3' Hp1a gRNA
Plasmid#127898PurposeWT Cas9 Vector targeting the 3' end of the mouse Hp1a geneDepositorInsertgRNA/Cas9
UseCRISPRAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
px330_SMAD exon 9 gRNA
Plasmid#68352Purposepx330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.DepositorInsertSMAD exon 9 gRNA
ExpressionMammalianPromoterU6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseExpressionBacterialPromoternative promoterAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantPromoterAtRPS5AAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only