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pFUW-Syb2-E78A

Plasmid

#248133

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with E78A mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-E78A (Vamp2 Rat)

Expression

Mammalian

Mutation

E78A

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-3AF

Plasmid

#245785

Purpose

Expression of mutated bovine arrestin-3 (I385A, V386A, F387A) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

I385A, V386A, F387A

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA3

Plasmid

#238988

Purpose

Mammalian expression of bovine arrestin-3 short isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-3 short (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA2-3A

Plasmid

#238991

Purpose

Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-2 long (ARRB2 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

I386A,V387A,F388A

Promoter

CMV

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtA3CL

Plasmid

#238992

Purpose

In vitro transcription of mutant bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

arrestin-3 short (ARR3 Bovine)

Use

In vitro transcription

Mutation

C17S, C60 V, C126S, C141L, C151V, C243V, C252V, C…

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-delta7

Plasmid

#234802

Purpose

Mammalian expression of bovine arrestin-3 (deleted residues 176, 177, 178, 182-185)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

deleted residues 176, 177, 178, 182-185

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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p3VEA3-3A

Plasmid

#234797

Purpose

Mammalian expression of bovine arrestin-3 (I385A,V386A,F387A)

Depositor

Vsevolod Gurevich

Article

Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

I385A,V386A,F387A

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTSK405

Plasmid

#190817

Purpose

For C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiae

Depositor

Tatiana Karpova, Gunjan Mehta

Article

Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

Insert

URA3 (URA3 Budding Yeast)

Use

Bacterial cloning vector

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSPObooster

Plasmid

#216160

Purpose

Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

Depositor

Grant Brown

Article

Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

Inserts

MKT1(30G) (MKT1 Budding Yeast)
RME1(ins-308A) (RME1 Budding Yeast)

Expression

Yeast

Mutation

MKT1 gene containing an aspartic acid to glycine …

Promoter

endogenous promoter

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-GAL-Kan

Plasmid

#232092

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUDE722

Plasmid

#103022

Purpose

expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHCAN1-4.S (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pUDE714

Plasmid

#103021

Purpose

expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHIS4-4.S (HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-d7F

Plasmid

#245784

Purpose

Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

deleted residues 175, 177, 178, 182-185

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA E45A

Plasmid

#217438

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA E45A

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA M66I

Plasmid

#217440

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA M66I

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pUDE724

Plasmid

#103023

Purpose

expression of a Cpf1 programming crRNA targetting PDR12 (crPDR12-3S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crPDR12-3.S (PDR12 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar4.3

Plasmid

#232743

Purpose

Expresses NADPH/NADP+ biosensor NAPstar4.3 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar4.3

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstarC

Plasmid

#232737

Purpose

Expresses control sensor NAPstarC in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstarC

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar3

Plasmid

#232740

Purpose

Expresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar3

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-ARS208a

Plasmid

#87383

Purpose

All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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