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p221a-GUS

Plasmid

#71263

Purpose

Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Beta-glucuronidase

Use

Gateway

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ash2L full

Plasmid

#15548

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Expression

Mammalian

Mutation

R622H (Please see depositor comments below)

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pMyc3ElbLuc

Plasmid

#53245

Purpose

reporter plasmid containing 3 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc3ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
Twitch-2B pcDNA3

Plasmid

#49531

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pHJ4: LexA-VHH-Caffeine

Plasmid

#108242

Purpose

LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with VHH-Caffeine antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA1

Plasmid

#26677

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 1 (KPNA1 Human)

Tags

T7

Expression

Mammalian

Mutation

KPNA1 sequence contains a reported SNP at S73N

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp PHAkt Cerulean

Plasmid

#50837

Purpose

Fluorescent reporter for membrane PIP3 levels

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PHAkt (AKT1 )

Use

Lentiviral and Synthetic Biology

Tags

Cerulean

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-NES

Plasmid

#64727

Purpose

Cytosol-targeted, FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

Insert

AKAR4-NES

Tags

Cerulean, NES, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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