165,528 results

1. pCDH-EF1-DIO-tdTomato

   Plasmid

   #72254

   Purpose

   Express tdTomato under EF-1 promoter when Cre is expressed by another vector

   Depositor

   Kazuhiro Oka

   Insert

   tdTomato
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD33-mf-lon

   Plasmid

   #21867

   Depositor

   Robert Sauer

   Article

   Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

   Insert

   Lon (Mfl404 Mesoplasma florum)
   

   Expression

   Bacterial

   Mutation

   all TGA codons changed to TGG codons

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. plc242-GFP-WDR24

   Plasmid

   #87050

   Purpose

   mammalian expression of WDR24

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   WDR24 (WDR24 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUP-(2xCouRS-tRNAopt)

   Plasmid

   #197930

   Purpose

   Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

   Inserts

   CouRS
   tRNA opt
   

   Expression

   Bacterial

   Promoter

   glnS' and lambda(ts) and proK

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CARPID dCasRx-BASU

   Plasmid

   #153303

   Purpose

   express CARPID dCasRx-BASU fusion protein in mammalian cells

   Depositor

   Jian Yan, Liang Zhang

   Article

   Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

   Insert

   dCasRx-BASU
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A in RfxCas13d

   Promoter

   EF-1a

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDN3.1(+)-CaNARi

   Plasmid

   #64848

   Purpose

   FRET-based calcineurin (CaN) activation biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSK659

   Plasmid

   #205128

   Purpose

   Expression of GFP-SnxA in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   SnxA
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRFP-Rab5

   Plasmid

   #14437

   Purpose

   Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 5 (RAB5A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_DLL1_WT

   Plasmid

   #82108

   Purpose

   Gateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DLL1 (DLL1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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