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pUb LwaCas13a + LwaCas13a Guide RNA

Plasmid

#176307

Purpose

Expresses LwaCas13a and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

LwaCas13a

Tags

HA tag

Expression

Insect

Mutation

E656G, I1060V, D1076G- please see depositor comme…

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab5 DN

Plasmid

#37694

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S43N (dominant negative)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Tags

His and myc

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
UAS-Xbp1-GFP

Plasmid

#80627

Purpose

expression of Xbp1-GFP (RA isoform) in Drosophila

Depositor

Pedro Domingos, Herman Steller

Article

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Tags

EGFP

Expression

Insect

Mutation

RA isoform

Promoter

UAS

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab5 CA

Plasmid

#37702

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q88L (constitutive active)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pAGN33

Plasmid

#131105

Purpose

An integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutants

Depositor

Riccardo Manganelli

Article

Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

Insert

P-furA102tetO-pip

Expression

Bacterial

Mutation

the furA102 promoter was mutated with the inserti…

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Plasmid

#169370

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Use

CRISPR

Expression

Insect

Promoter

CPIJ039596

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab11 WT

Plasmid

#37711

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pCI-Flag-NEDD4L∆WW2

Plasmid

#166708

Purpose

expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W453A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 453 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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