We narrowed to 22,283 results for: Fus
-
Plasmid#203810PurposePlasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6_PT1
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-Cas9-VPR
Plasmid#115147PurposeSafe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassetteDepositorInsertBlastR-P2A-Cas9-VPR
UseCRISPRTagsSV40NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI K270A
Plasmid#167290PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationK270APromoterSFFVAvailable sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0005
Plasmid#42245PurposegRNA targeted to zebrafish gene gsk3bDepositorInsertgRNA-gsk3b (gsk3b Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-T2A-miniSOG-C-Far
Plasmid#193915PurposeExpresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV productionDepositorInsertminiSOG
UseAAVTagsFarnesylation signal sequence and Flag tagExpressionMammalianMutationPromoterhSynAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC-H6
Plasmid#183777PurposeExpress PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0001
Plasmid#42241PurposegRNA targeted to zebrafish gene apoeaDepositorInsertgRNA-apoea (apoea Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#199453PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B geneDepositorInsertHDR donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 UPA-STOP
Plasmid#164031PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPADepositorInsertCARD8 (CARD8 Human)
UseTagsHis6-MBP-3CExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
IDA3::HA exon2 (WS13)
Plasmid#126874Purposep3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::NeonGreen N-term (WS14)
Plasmid#126875PurposeNeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherTagsNeonGreenExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::NeonGreen exon2 (WS15)
Plasmid#126876PurposeNeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer20
Plasmid#44012PurposeTet-inducible lentiviral vector for ORF expressionDepositorTypeEmpty backboneUseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF3550
Plasmid#145026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1788
Plasmid#143847PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCERG1 (TCERG1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorInsertWilms tumor 1 associated protein (WTAP Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-flag-YTHDF2
Plasmid#52300Purposefull length YTHDF2 with N-terminal flag tag for mammalian cell expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsflagExpressionMammalianMutationPromoterT7Available sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-flag-YTHDF2-N
Plasmid#52302PurposeFlag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)DepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsflagExpressionMammalianMutationChanged E384 to stop codonPromoterT7Available sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-flag-YTHDF2-C
Plasmid#52303PurposeFlag-tagged YTHDF2 C-terminal domain, from E384 to the endDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsflagExpressionMammalianMutationDeleted amino acids 1-383PromoterT7Available sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only