We narrowed to 15,378 results for: NTS

Showing: 1521 - 1540 of 15378 results

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pCKC776

Plasmid

#226627

Purpose

piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
EF1a promoter, mCherry

Use

Synthetic Biology

Tags

oxygen dependent degron (ODD)

Expression

Mammalian

Promoter

4xHRE_YB TATA and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts

Plasmid

#195065

Purpose

Expresses FLAG-tagged Drosophila mts protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Pp2A-29B

Plasmid

#195066

Purpose

Expresses FLAG-tagged Drosophila Pp2A-29B protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Protein phosphatase 2A at 29B (Pp2A-29B Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMDC32-FIT2-FLL[157-159]AAA

Plasmid

#96991

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Expression

Plant

Mutation

Amino acid residues 157-159 were mutated (FLL[157…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-SNAP-hCB2_IRES_EGFP

Plasmid

#223506

Purpose

Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Article

Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

Insert

SNAP-tag-CNR2 and EGFP (CNR2 Human, Synthetic)

Tags

SNAP-tag

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts D85N

Plasmid

#208403

Purpose

Expresses FLAG-tagged Drosophila mts protein with D85N mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

D85N

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H59Q

Plasmid

#208402

Purpose

Expresses FLAG-tagged Drosophila mts protein with H59Q mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

H59Q

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H241A

Plasmid

#208404

Purpose

Expresses FLAG-tagged Drosophila mts protein with H241A mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

H241A

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMDC43-FIT2-FLL[157-159]AAA

Plasmid

#96994

Purpose

Express GFP-tagged mutated mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Tags

GFP

Expression

Plant

Mutation

Amino acid residues 157-159 were mutated (FLL[157…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCX-DI-2A

Plasmid

#74671

Purpose

Mammalian expression of DI-2A (hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

Inserts

CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMHE-membrane-mEGFP

Plasmid

#105526

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

GNAI2 (GNAI2 Human)

Use

In vitro transcription

Tags

mEGFP

Mutation

fragment encoding the plasma membrane targeting s…

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT.2#2/Cre

Plasmid

#173661

Purpose

Expresses a NT.2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-ArcΔCTD

Plasmid

#119878

Purpose

Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Tags

GST

Expression

Bacterial

Mutation

Deletion of nt 832-1110

Promoter

tac

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.mNeon

Plasmid

#69146

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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hum alpha ENAC promoter a25-a23

Plasmid

#83427

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

Insert

human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)

Use

Luciferase

Tags

Luciferase

Promoter

alpha ENAC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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lentiMutB3GNT5-blast

Plasmid

#208380

Purpose

lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

nt 922 c->g (PAM site inactivation)

Promoter

EF-1 alpha core promoter

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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lentiMutB3GALT5-blast

Plasmid

#208381

Purpose

lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

beta-1,3-galactosyltransferase 5 (B3GALT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

nucleotide change in PAM targeting sequence nt 51…

Promoter

EF-1 alpha core promoter

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT.3#3/Cre

Plasmid

#173662

Purpose

Expresses a NT.3-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-1

Plasmid

#133353

Purpose

human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

Plasmid

#138525

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

Depositor

Richard Jenner

Article

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

Control non-PRC2 binding A-tract repeat RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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