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pcDNA6-C-3XFLAG-h-Lmbr1l

Plasmid

#123462

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Limb development membrane protein 1 like (LMBR1L Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Axin1

Plasmid

#123463

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Axin 1 (Axin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-N-HA-Gp78

Plasmid

#123591

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

autocrine motility factor receptor (Amfr Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Gp78

Plasmid

#123590

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

autocrine motility factor receptor (Amfr Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RVR

Plasmid

#108863

Purpose

FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R, K629R and N633R, Cpf1 is rice codon optimi…

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-3XFLAG-m-Lmbr1l

Plasmid

#123593

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

limb region 1 like (Lmbr1l Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-HA-m-Lmbr1l

Plasmid

#123594

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

limb region 1 like (Lmbr1l Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

Plasmid

#198845

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-R18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro

Plasmid

#198841

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-R15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

Plasmid

#198840

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-L17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

Plasmid

#198844

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-L18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBT120a17-sg-del

Plasmid

#214347

Purpose

Expresses pIII

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

gIII-xLuxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iAChSnFR

Plasmid

#137955

Purpose

Expresses iAChSnFR under CAG promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Has Service

AAV1 and AAV9

Insert

iAChSnFR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Lrp6

Plasmid

#123595

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Gsk3b

Plasmid

#123592

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

glycogen synthase kinase 3 beta (Gsk3b Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Ubxd8

Plasmid

#123599

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Fas associated factor family member 2 (Faf2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iAChSnFR

Plasmid

#137950

Purpose

Expresses iAChSnFR under hSynapsin promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Has Service

AAV1

Insert

iAChSnFR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynanpsin

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iAChSnFR-NULL

Plasmid

#137951

Purpose

Expresses iAChSnFR (non-binding) under hSynapsin promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR (non-binding variant)

Use

AAV

Tags

Expression

Mammalian

Mutation

Y140A

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.ZAC.GFAP.iAChSnFR

Plasmid

#137962

Purpose

Expresses iAChSnFR under GFAP promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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