We narrowed to 6,740 results for: tac

1. pGEX-6P-1-INTS3-FL

   Plasmid

   #128415

   Purpose

   Express INTS3 full-length in E. coli with a GST tag (N-terminal)

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 (INTS3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   full-length

   Promoter

   tac promoter

   Available since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

   Plasmid

   #108693

   Purpose

   For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Inserts

   RNASEH2B (RNASEH2B Human)
   RNASEH2C (RNASEH2C Human)
   RNASEH2A-D34A/D169A
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Shine Dalgarno sequence upstream of start codon, …

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX4T1_GST-PLproCS-MBP

   Plasmid

   #169195

   Purpose

   To express a PLpro substrate (GST-PLproCS-MBP) in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   GST-PLproCS-MBP
   

   Use

   Tags

   GST-MBP fusion (PLpro-cleavable) and His6 (intern…

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGex-4T-2_LC3A

   Plasmid

   #73946

   Purpose

   For recombinant expression of human LC3A/MAP1LC3A in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   LC3A (MAP1LC3A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pGEX PLCg1(NC)-SH2

   Plasmid

   #46471

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg1(NC) (PLCG1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 542-759

   Promoter

   Tac

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX Grb2-SH2

   Plasmid

   #46440

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grb2 (GRB2 Human)
   

   Use

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 58-159

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX FynA-SH2

   Plasmid

   #46429

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   FynA (FYN Human)
   

   Use

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 142-251

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX Stat3-SH2

   Plasmid

   #46513

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat3 (STAT3 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 584-700

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX6P2-MDMX (23-111)

   Plasmid

   #62064

   Purpose

   Expression of human MDM4 (residues 23-111) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 23-111

   Promoter

   tac

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGEX Grb2 SH2-SH3

    Plasmid

    #46442

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb2 (GRB2 Human)
    

    Use

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 1-217

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HTRA1_PDZ_1

    Plasmid

    #103912

    Purpose

    Protein expression and purification of HTRA1 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    HTRA1 (HTRA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pNSSCP

    Plasmid

    #183188

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DLG4_PDZ_3

    Plasmid

    #103953

    Purpose

    Protein expression and purification of PSD95 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG4 (DLG4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGex-4T-2_GEC1

    Plasmid

    #73945

    Purpose

    For recombinant expression of human GEC1/GABARAPL1 in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    GEC1 (GABARAPL1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pGEX Fer-SH2

    Plasmid

    #46425

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Fer (FER Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 450-558

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6P2-MDMX (1-120)

    Plasmid

    #62065

    Purpose

    Expression of human MDM2 (1-120) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 1-120

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGEX SHP-1(C)-SH2

    Plasmid

    #46494

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(C) (PTPN6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 105-217

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX Hck-SH2

    Plasmid

    #46445

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Hck (HCK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 134-254

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Stat1-SH2

    Plasmid

    #46511

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat1 (STAT1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 488-683

    Promoter

    Tac

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge