Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,972 results for: try

Clear keywords
Showing: 1521 - 1540 of 2972 results

  5. pCI-Flag-NEDD4L∆WW2

    Plasmid
    #166708
    Purpose
    expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEDD4L W453A
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 453 to alanine
    Promoter
    CMV promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166708-plasmid-map-sequence-id-329114

    Enlarge

  6. pCI-Flag-NEDD4L∆WW3

    Plasmid
    #166709
    Purpose
    expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEDD4L W525A
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 525 to alanine
    Promoter
    CMV promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166709-plasmid-map-sequence-id-329115

    Enlarge

  9. pENTR-AtTAS1c-D2-B/c

    Plasmid
    #137883
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Publication
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Use
    Gateway-compatible entry clone
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    137883-plasmid-map-sequence-id-275808

    Enlarge

  16. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Depositor
    Henry Lester, Jerry Stitzel
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    48811-plasmid-map-sequence-id-193963

    Enlarge

  18. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113687
    Purpose
    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113687-plasmid-map-sequence-id-231825

    Enlarge

  19. FGFR2 N549K 3xFlag

    Plasmid
    #110107
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Asparagine 549 to Lysine (N549K)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110107-plasmid-map-sequence-id-209777

    Enlarge

  20. Epac-S-H171

    Plasmid
    #170353
    Purpose
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170353-plasmid-map-sequence-id-345820

    Enlarge

Showing: 1521 - 1540 of 2972 results