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pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Flag- MCL1-3A (S159, S162, T163 mutated to A)

Plasmid

#197458

Purpose

MCL-1 phosphodegron mutants

Depositor

Omar Khan

Article

Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

Insert

MCL1 (MCL1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S159A, S161A, T163A

Promoter

CMV

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Basigin-isoform2-CD4d3+4-bio

Plasmid

#73129

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin isoform 2 (BSG Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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miniCGBE1 (pBM1219)

Plasmid

#140253

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pG2K

Plasmid

#71742

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-li-p33_RS-CLIP

Plasmid

#104634

Purpose

Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

Depositor

Sébastien Walchli

Article

Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

Insert

modified Invariant chain p33 (CD74 Human)

Use

Entry vector for gateway system

Tags

none

Mutation

CLIP domain was replaced by 2 restriction sites

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQAT57

Plasmid

#223429

Purpose

T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot6

Plasmid

#37368

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot6 (CNOT6 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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CAP-AAV.FUS.3

Plasmid

#222333

Purpose

AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

Depositor

Mikhail Shapiro, Jerzy Szablowski

Article

Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

Insert

AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1

Use

AAV

Promoter

p41

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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