We narrowed to 1,591 results for: PTS

1. pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

   Plasmid

   #208873

   Purpose

   For expression in E. coli of NAP1 (delta-TBK1)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

   Plasmid

   #208863

   Purpose

   Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   S37K/A44E (disrupts NDP52 binding)

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

   Plasmid

   #208864

   Purpose

   Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   I11S/L12S (disrupts FIP200 binding)

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

   Plasmid

   #208865

   Purpose

   Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET41sIII_GST-ALC1-K77R

   Plasmid

   #174813

   Purpose

   Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-K77R (CHD1L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K77R

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET41sIII_GST-ALC1-E175Q

   Plasmid

   #174814

   Purpose

   Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-E175Q (CHD1L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E175Q

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLB(N)CX-FLAG-CH3 Del33

   Plasmid

   #133724

   Purpose

   Retroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

   Depositor

   James A. DeCaprio

   Article

   Borger et al J Virol. 2006 May . 80(9):4292-303.

   Insert

   EP300 (EP300 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mutation

   partial (not complete) p300 fragment, AT and CH3 …

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

   Plasmid

   #83458

   Purpose

   Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Amino-acids 4127-4303, S4144D

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. HiBiT-FZD4

   Plasmid

   #195842

   Purpose

   Expresses human FZD4 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD4 (FZD4 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HiBiT-FZD2

    Plasmid

    #195840

    Purpose

    Expresses human FZD2 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD2 (FZD2 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-hMC4R

    Plasmid

    #179315

    Purpose

    Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    melanocortin 4 receptor (MC4R Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HiBiT-FZD6

    Plasmid

    #195844

    Purpose

    Expresses human FZD6 with an N-terminal HiBiT; contains M345L

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD6 (FZD6 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    M345L

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. KAT6B-Tol2

    Plasmid

    #113384

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

14. KAT6B-luciferase

    Plasmid

    #113353

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HiBiT-FZD9

    Plasmid

    #195847

    Purpose

    Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD9 (FZD9 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-HDC-DIO-GFP-shRNA.scramble

    Plasmid

    #184331

    Purpose

    Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    histidine decarboxylase promoter fragment that g…

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav2.2 D122A HA pCAGGS

    Plasmid

    #206097

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV/B-actin

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.2 HA D122A pcDNA3

    Plasmid

    #206108

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

    Plasmid

    #80640

    Purpose

    Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed Glutamate 209 to alanine

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. cacna2d1 R241A pMT2

    Plasmid

    #206110

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R241A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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