We narrowed to 1,891 results for: ogn

1. pGWB514-35S:FLS2XL-HA

   Plasmid

   #226412

   Purpose

   Expressing Vitis riparia FLS2XL (FLS2 with eXtended Ligand recognition) protein in plants

   Depositor

   Gitta Coaker

   Insert

   FLS2XL
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-Eco31I

   Plasmid

   #165506

   Purpose

   Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Eco31I
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pOpen-PstIM

   Plasmid

   #165536

   Purpose

   Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   M.PstI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pOpen-EcoRIM

   Plasmid

   #165515

   Purpose

   Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   M.EcoRI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pBD027

   Plasmid

   #201310

   Purpose

   avrSr27-3 as Golden Gate compatible MoClo CDS part (no recognition by Sr27)

   Depositor

   Benjamin Schwessinger

   Insert

   avrSr27-3
   

   Use

   Tags

   Expression

   Plant

   Mutation

   removal of BsaI and BpiI sites

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOpen-SfiIR (cDNA)

   Plasmid

   #165528

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   SfiI (used to create cDNA libraries)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAGGS-ROSAf

   Plasmid

   #54323

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-ROSAr

   Plasmid

   #54324

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-DNA Topoisomerase 1B Vaccinia Virus

   Plasmid

   #165510

   Purpose

   Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DNA Topoisomerase 1B Vaccinia Virus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. PPI63

    Plasmid

    #25197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ppi63.0007.0179 (NKTR Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. NKTR

    Plasmid

    #25597

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NKTR (NKTR Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pMS2-1

    Plasmid

    #220627

    Purpose

    Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZE-P9-egfp

    Plasmid

    #201790

    Purpose

    the promoter can be recognized by PadR

    Depositor

    Yajun Yan

    Article

    Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

    Insert

    P9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS2-2

    Plasmid

    #220628

    Purpose

    Plasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p_SNIPR_target_WT

    Plasmid

    #139465

    Purpose

    T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    Target_WT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p_SNIPR_target_mut_36

    Plasmid

    #139466

    Purpose

    T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    Target_mut36
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH03C_32

    Plasmid

    #120897

    Purpose

    Functional test of ECF sigma factors and their cognate promoters

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

    Insert

    ECF32_1122
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p_SNIPR_target_GUmut33AtoG

    Plasmid

    #139468

    Purpose

    T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 33

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    Target_mutGU33
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-(empty)-TAG

    Plasmid

    #138209

    Purpose

    To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Type

    Empty backbone

    Use

    T7 transcription

    Tags

    TAG sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter, T7 Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNR188

    Plasmid

    #79543

    Purpose

    Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge