We narrowed to 22,825 results for: Sis

1. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Available Since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. VAMP2-pHmScarlet

   Plasmid

   #166890

   Purpose

   For vesicle exocytosis visualization using pHmScarlet

   Depositor

   Pingyong Xu

   Article

   Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

   Insert

   VAMP2 (Vamp2 Mouse)
   

   Tags

   pHmScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFGC5941-PacI-ath-STTM832-5p

   Plasmid

   #84163

   Purpose

   target miRNA832-5p for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM832-5p
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFGC5941-PacI-ath-STTM832-3p

   Plasmid

   #84162

   Purpose

   target miRNA832-3p for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM832-3p
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDB179-DuraPETase

   Plasmid

   #218699

   Purpose

   Encodes codon optimized DuraPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DuraPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCK054 pDEST exorh:mCerulean

   Plasmid

   #195985

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK017 pDEST exorh:EGFP

   Plasmid

   #195983

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST exorh:mCherry

   Plasmid

   #195989

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann, Nicolas Rohner

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Plasmid L5 attB::Pleft* mScarlet

   Plasmid

   #169410

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mScarlet proper
   

   Use

   Unspecified

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Plasmid L5 attB::Pleft* mWasabi

    Plasmid

    #169409

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mWasabi
    

    Use

    Unspecified

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. MP6-SlugABEN-738

    Plasmid

    #214348

    Purpose

    Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
    

    Expression

    Bacterial

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Xenopus APC-myc

    Plasmid

    #16686

    Depositor

    Barry Gumbiner

    Article

    Vleminckx et al J Cell Biol. 1997 Jan 27. 136(2):411-20.

    Insert

    APC (apc.L Frog)
    

    Use

    Xenopus expression

    Tags

    Myc

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pENTR5'-attP (pCM254)

    Plasmid

    #68311

    Purpose

    Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    attP
    

    Use

    Gateway entry vector

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPR v2-sgControl

    Plasmid

    #230080

    Purpose

    Crispr knock out control

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    non-specific sequence control
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMpGE013

    Plasmid

    #108681

    Purpose

    All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKI1.1R

    Plasmid

    #85808

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Plant

    Promoter

    AtRPS5A

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK02

    Plasmid

    #105133

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKanB

    Plasmid

    #223523

    Purpose

    Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. IRE1-2EGFP-ter-kanMX4

    Plasmid

    #184766

    Purpose

    Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    IRE1 (IRE1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 5'-myr-dEGFP-3'

    Plasmid

    #16075

    Depositor

    Erin Schuman

    Article

    Aakalu et al Neuron. 2001 May . 30(2):489-502.

    Insert

    Protein synthesis reporter (Camk2a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCDB179-HotPETase

    Plasmid

    #218701

    Purpose

    Encodes codon optimized HotPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    HotPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. plRL2

    Plasmid

    #163631

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-Mem

    Plasmid

    #55779

    Purpose

    This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    mCherry-Mem
    

    Tags

    The N-terminal 20 residues of neuromodulin are fu…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSUPER-scramble

    Plasmid

    #118349

    Purpose

    This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    scrambled shRNA
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKIR1.1

    Plasmid

    #85758

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Plant

    Promoter

    AtRPS5A

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-EF1alpha-dCas9-p300_Blast

    Plasmid

    #192653

    Purpose

    3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUB-cSypHer

    Plasmid

    #84733

    Purpose

    Expresses SypHer in plant cells-biosensor for pH

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    SypHer
    

    Expression

    Plant

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRPF215

    Plasmid

    #106377

    Purpose

    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

    Insert

    Ptet-himar1-Ter(slpA)-transposon
    

    Use

    E. coli - c. difficile shuttle vector

    Mutation

    Codon optimised for C. difficile

    Promoter

    Ptet

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDB179-DepoPETase

    Plasmid

    #218702

    Purpose

    Encodes codon optimized DepoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DepoPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDB179-ThermoPETase

    Plasmid

    #218698

    Purpose

    Encodes codon optimized ThermoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    ThermoPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHEE401_GV_MOD

    Plasmid

    #194874

    Purpose

    mCherry selection in Arabidopsis seeds in T1 and T2

    Depositor

    Wolfgang Busch

    Article

    Ruiz Rosquete et al Plant J. 2025 Jan;121(1):e17177. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Promoter

    egg cell-specific

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

    Plasmid

    #192650

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-VP64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

    Plasmid

    #192651

    Purpose

    3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-10xGCN4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-basP:fLuc-TK:rLuc

    Plasmid

    #138369

    Purpose

    Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAGC-Lb12-P1Bb

    Plasmid

    #231387

    Purpose

    For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    U6-29
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge