We narrowed to 2,550 results for: CCS

1. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97309

   Purpose

   HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HR donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPV00605 pET21GG2-His(6)-BECN(1-265)

   Plasmid

   #136686

   Purpose

   Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 IDR + CC (BECN1 Human)
   

   Use

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-KLF4

   Plasmid

   #192908

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-UBC-S1-jRCaMP1a

   Plasmid

   #160728

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S1-jRCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-cT3G-cERP2-JUN

   Plasmid

   #192895

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of JUN

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   JUN (JUN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97310

   Purpose

   NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb NHEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-crRNA(TARDBP)

   Plasmid

   #109323

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (TARDBP Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. XE129 beta2AR Fz1-pcDNA3.1

   Plasmid

   #16795

   Depositor

   Randall Moon

   Article

   Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

   Insert

   beta 2 AR Fz1 (Fzd1, Adrb2 Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Chimera of beta 2 adrenergic receptor (extracellu…

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pJJR50

   Plasmid

   #75026

   Purpose

   U6 promoter driven flipped + extended sgRNA expression vector

   Depositor

   Mike Boxem

   Article

   Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

   Insert

   guide RNA, flipped and extended version
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   R07E5.16 (U6)

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mPC1_4077-eGFP-3HA

    Plasmid

    #108390

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mPC1_4119-3HA

    Plasmid

    #108395

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-SAM-Puro

    Plasmid

    #198713

    Purpose

    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-SAM-Cre

    Plasmid

    #198714

    Purpose

    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pgk and U6

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgRNA-Cre-GpNLuc-sgmMRE11

    Plasmid

    #208384

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgMRE11 (Mre11a Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3

    Plasmid

    #208389

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgZBP1 (Zbp1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-sgRNA-Cre-GpNLuc-sgmMLKL

    Plasmid

    #208391

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgMLKL (Mlkl Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mouse ZNG1

    Plasmid

    #197399

    Purpose

    expresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)

    Depositor

    David Giedroc

    Article

    Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

    Insert

    ZNG1 (Cbwd1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUMO mouse METAP1

    Plasmid

    #197395

    Purpose

    Expresses SUMO-tagged mouse METAP1

    Depositor

    David Giedroc

    Article

    Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

    Insert

    METAP1 (Metap1 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-mPC1_4119-eGFP-3HA

    Plasmid

    #108393

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-mPC1_4077-3HA

    Plasmid

    #108392

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge