We narrowed to 2,675 results for: CCS

Showing: 1541 - 1560 of 2675 results

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TPC1(ΔN69)-mCherry

Plasmid

#135184

Purpose

TPC1 with truncated N-terminus, tagged with mCherry on C terminus.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 1-69

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor

Plasmid

#97318

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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TPC2(ΔN49)-mCherry

Plasmid

#135185

Purpose

TPC2 with truncated N-terminus, tagged with mCherry on C terminus.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 1-49

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL210

Plasmid

#126451

Purpose

Expresses Cas9-XTEN-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor

Plasmid

#97316

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor

Plasmid

#97315

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTBL680 CHYRON3 integration construct

Plasmid

#126448

Purpose

To integrate the CHYRON3 locus at AAVS1 in human cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6/3xLacO-CHYRON3 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6/3xLacO

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

Plasmid

#200977

Purpose

Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

D62Q-Q130L-V167L (increase specificity towards he…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

Plasmid

#200980

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

Plasmid

#117856

Purpose

This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-Halo-hMRE11(H129N)

Plasmid

#208394

Purpose

To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Mutation

H129N

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn1-AsCpf1(RR)

Plasmid

#109325

Purpose

AAV vector expressing AsCpf1 (RR variant)

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

hAsCpf1(RR)

Use

AAV

Tags

HA and NLS

Mutation

S542R/K607R

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ELK1

Plasmid

#192918

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMB41

Plasmid

#73087

Purpose

N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

Avi-GFP tag

Tags

Avi and GFP

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-PLXNB2-mGAP

Plasmid

#86241

Purpose

Lentivirus for expression of human Plexin-B2 with mutant GAP domain

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PLXNB2 with point mutations of GAP domain

Promoter

hPGK

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMB72

Plasmid

#73088

Purpose

C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

GFP-Avi tag

Tags

GFP and Avi

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMB71

Plasmid

#73085

Purpose

Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTBL469

Plasmid

#126458

Purpose

Expresses Cas9-15xFlag-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

Cas9
15x Flag
TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJHG003

Plasmid

#126454

Purpose

Expresses Cas9-XTEN-dTdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

Added D343E and D345E to inactivate TdT.

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMB37

Plasmid

#73084

Purpose

Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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