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pDonor-Prf1-mock-ires-mCherry

Plasmid

#209074

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKT-H3.3-G34R-IRES-katushka

Plasmid

#209428

Purpose

SB-transposable plasmid expressing the mutant histone H3.3-G34R with RFP

Depositor

Maria Castro

Insert

H3.3-G34R

Use

Tags

Expression

Mammalian

Mutation

G34R

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynW pHluorin-SYT9 IRES mRuby3

Plasmid

#195698

Purpose

Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C ACT-DCC-Halo

Plasmid

#195148

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove act…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C ACT-DCC-GFP

Plasmid

#195147

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove act…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pb-miRE-tre-3xFLAG-hnRNPU∆RGG

Plasmid

#196088

Purpose

Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAF-A (Hnrnpu Mouse)

Use

Tags

N-terminal 3xFLAG

Expression

Bacterial and Mammalian

Mutation

removed last 154 amino acids on cterminal end

Promoter

TRE

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD 3R-3E

Plasmid

#188148

Purpose

Expresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD 3E@3R

Plasmid

#188149

Purpose

Expresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD 3E-3R

Plasmid

#188147

Purpose

Expresses C-terminal flag-tagged human CAD 3E-3R in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17

Plasmid

#193299

Purpose

tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Klf4-IRES-Sox2-17 (Sox2, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRps9-D95N-IRES-GFP-HygR

Plasmid

#190272

Purpose

Express RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

Depositor

Erik Böttger

Article

Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

Insert

ribosomal protein RPS9

Use

Tags

IRES-eGFP

Expression

Mammalian

Mutation

D95N

Promoter

CAGGS-CMV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-Fzd1-IRES-tdTomato (TET006)

Plasmid

#160510

Purpose

This plasmid expresses Fzd1 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al bioRxiv 2022

Insert

Fzd1 (Fzd1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190080

Purpose

express cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-rabbit-Caspase-4

Plasmid

#183375

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-caspase-1/4a

Plasmid

#183393

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of human caspase-1 fused to catalytic domain of human caspase-4 (CASP1 Synthetic, Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-cat-Caspase-1/4b

Plasmid

#183366

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4b

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-mSNCA(1-95)

Plasmid

#185716

Purpose

AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (Snca Mouse)

Use

AAV

Tags

Expression

Mutation

deleted AA 95-144

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP-horse-Caspase-4

Plasmid

#183380

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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