We narrowed to 2,785 results for: cdna expression plasmid

1. pFRT-TODestFLAGHA_RNAseH1

   Plasmid

   #65782

   Purpose

   expresses FLAGHA-tagged RNAsH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-DmPABPC1-V5His6_D

   Plasmid

   #146093

   Purpose

   Insect Expression of DmPABPC1

   Depositor

   Elisa Izaurralde

   Insert

   DmPABPC1 (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non-silent mutation G26S compared to the sequ…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDZ2FE_Champ1_1-812_2PA

   Plasmid

   #105676

   Purpose

   Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding

   Depositor

   Thanos Halazonetis

   Insert

   Champ1 (CHAMP1 Human)
   

   Use

   Tags

   2X Flag tag

   Expression

   Mammalian

   Mutation

   Mutations: P336A, P341A

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVH41-Tier1-PhCMV-GLuc

   Plasmid

   #169544

   Purpose

   Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Gaussia Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH608-Tier1-PmPGK1-Fluc

   Plasmid

   #169543

   Purpose

   Tier-1 vector encoding PmPGK1-driven Fluc (PmPGK1-Fluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven Firefly Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT-TODestGFP_RNAseH1

   Plasmid

   #65784

   Purpose

   expresses GFP-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFRT-TODestRFP_RNAseH1

   Plasmid

   #65785

   Purpose

   expresses RFP-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   RFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFRT-DestFLAGHA_RNAseH1

   Plasmid

   #65783

   Purpose

   constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA Display H3_A/Hong Kong/1/1968

   Plasmid

   #206506

   Purpose

   Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells

   Depositor

   IIya Finkelstein

   Insert

   H3_A/Hong Kong/1/1968
   

   Use

   Tags

   3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

   Expression

   Mammalian

   Mutation

   deleted amino acids 1651 to 1735

   Promoter

   CMV

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-EGFP-DmMTR-4

    Plasmid

    #66591

    Purpose

    expresses EGFP-tagged MTR-4 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    MTR-4 (l(2)35Df Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Actin 5C

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cx43 delta dt GFP

    Plasmid

    #17671

    Depositor

    Cecilia Lo

    Insert

    Cx43 (Gja1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    a.a. 234-243 deleted

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pUASp YFP Ras64B DN

    Plasmid

    #52243

    Purpose

    Drosophila expression of YFP-Ras64B dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Ras64B (Ras64B Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S19N; DN mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pUASp YFP Ras64B CA

    Plasmid

    #52244

    Purpose

    Drosophila expression of YFP-Ras64B constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Ras64B (Ras64B Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q63L; CA mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pAc5.1B-lambdaN-DmPABPC1-V5His6_D

    Plasmid

    #146095

    Purpose

    Insect Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    DmPABPC1 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    seven silent mutations compared to the sequence g…

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RF-227-UB-BMX

    Plasmid

    #79186

    Purpose

    Expression of the C-terminal fragment of BMX that is endogenously generated by caspase cleavage in mammalian cells

    Depositor

    Richard Fahlman

    Insert

    BMX (BMX Human)
    

    Use

    Tags

    3x Flag and ubiquitin

    Expression

    Mammalian

    Mutation

    delta 1-242

    Promoter

    CMV

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICE-FLAG-NAT10-siR-WT

    Plasmid

    #59365

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    human NAT10 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutations to render cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICE-FLAG-NAT10-siR-G641E

    Plasmid

    #59366

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    NAT10 NM_024662 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    G641E and silent mutations to render the cDNA res…

    Promoter

    CMV-tet

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BcatMutS33/S37.T41/S45

    Plasmid

    #24204

    Depositor

    David Rimm

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S33A, S37A, T41A,S45A

    Promoter

    CMV

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tau (P301L)-VC

    Plasmid

    #87633

    Purpose

    expresses hTau fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Mutation P301L

    Promoter

    CMV

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. VN-Tau (P301L)

    Plasmid

    #87634

    Purpose

    expresses hTau fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Mutation P301L

    Promoter

    CMV

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge