We narrowed to 2,718 results for: fas

1. pcDNA-NBid-PhoCl2c-CBid

   Plasmid

   #164051

   Purpose

   Optogenetic manipulation of cell apoptosis using PhoCl2c

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NBid-PhoCl2c-CBid
   

   Tags

   kozak sequence

   Expression

   Mammalian

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC1430

   Plasmid

   #127981

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ACPPiso1-V5

   Plasmid

   #113052

   Purpose

   Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

   Insert

   prostatic acid phosphatase isoform 1 (ACP3 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACPPiso2-V5

   Plasmid

   #113053

   Purpose

   Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

   Insert

   prostatic acid phosphatase isoform 2 (ACP3 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRU325

   Plasmid

   #167676

   Purpose

   Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE1109

   Plasmid

   #202773

   Purpose

   CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:TREX2-zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCyterm-mScarlet-I3_N1

   Plasmid

   #189777

   Purpose

   Construct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assay

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   Cyterm-mScarlet-I3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

   Plasmid

   #182009

   Purpose

   CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   IgKchL_HA_STf_myc_PDGFRtmb
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-VARNAM

    Plasmid

    #115554

    Purpose

    Red fluorescent voltage indicator for neuronal expression

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

    Insert

    VARNAM
    

    Use

    AAV

    Tags

    Golgi and ER export signal

    Promoter

    human Synapsin

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Promoter

    Z3EV

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

    Plasmid

    #20946

    Purpose

    3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    halorhodopsin
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_NES_STf

    Plasmid

    #182001

    Purpose

    CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    NES-STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE1108

    Plasmid

    #202772

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL2013

    Plasmid

    #172182

    Purpose

    A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

    Depositor

    Kan Wang

    Article

    McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

    Insert

    mCherry
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MMLV-CAG-G_oT7pol-IRES-BSD

    Plasmid

    #172369

    Purpose

    Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oT7pol + BSD
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-myrSNAP

    Plasmid

    #60098

    Purpose

    Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional

    Depositor

    Gregory Jefferis

    Article

    Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

    Inserts

    SNAP-tag
    histone-GFP
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. eGFP-CBP

    Plasmid

    #169211

    Purpose

    Expression of eGFP-CBP protein for chitin visualization

    Depositor

    James S Fraser

    Article

    Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

    Insert

    Chitinase A1 Chitin Binding Domain
    

    Tags

    6xHis, Mxe Gyr Intein, and eGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExpreS2-1

    Plasmid

    #175360

    Purpose

    ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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