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PMT-mApple_2

Plasmid

#203772

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

PMT-mApple dimer

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pWT007a

Plasmid

#107889

Purpose

W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWT047c

Plasmid

#107903

Purpose

W2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PR-sd5-BE2, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT045c

Plasmid

#107902

Purpose

W2.6 plasmid. Contains PTetO-SD8-BE2, PPSP-sgRNA1 and is part of CAMERA 2.6

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PPSP-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007g

Plasmid

#107890

Purpose

W1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007h

Plasmid

#107892

Purpose

W1.0.1c plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA(con.) and is part of CAMERA 1.0.1c

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA(con.)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT060b

Plasmid

#107904

Purpose

W2m.0-2 plasmid. Contains U6-sgRNA A (CCR5) and is part of a system for CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA A (CCR5) (CCR5 Human)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Tags

GFP

Expression

Worm

Promoter

eft-3

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-Cre

Plasmid

#50797

Purpose

Expression of Cre recombinase under the control of the UAS-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Cre recombinase

Use

Multi-species

Promoter

UAS-hsp70

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pRoTnsABCDQ

Plasmid

#178772

Purpose

Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRoDonor

Plasmid

#178773

Purpose

Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-PacI

Plasmid

#170100

Purpose

deGFP reporter plasmid with unique PacI restriction enzyme recognition site

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA1

Plasmid

#170088

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas7

Plasmid

#170092

Purpose

expresses E. coli type I-E Cas7

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas7

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas8

Plasmid

#170093

Purpose

expresses E. coli type I-E Cas8

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCse2

Plasmid

#170094

Purpose

expresses E. coli type I-E Cse2

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cse2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_dimer_mKate2

Plasmid

#167333

Purpose

Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K95A, Y99F, E106A, E112A and R116A

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_mKate2

Plasmid

#167334

Purpose

Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) and HMGA …

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_dHMGA_mKate2_CLEAVAGE

Plasmid

#167337

Purpose

Expresses mKate2 fused to TFAM dHMGA mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTK729

Plasmid

#114710

Purpose

Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705+1700-2115ΔNLS)-RFP-Nano

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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