We narrowed to 5,328 results for: pcdna3.1

1. pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

   Plasmid

   #50031

   Purpose

   Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   PM-SrtA(strep)
   

   Tags

   C-HA

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-2xFLAG-2xSTREP-CDK4

   Plasmid

   #172615

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

   Plasmid

   #127322

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)Gαq-124-NLuc

   Plasmid

   #207153

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAQ (GNAQ Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(124)E

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

   Plasmid

   #207160

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNG2 (GNG2 Human)
   

   Expression

   Mammalian

   Mutation

   N-terminally tagged with LSS-mKATE2

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP Rab11a WT pcDNA3.1 Zeo (+)

   Plasmid

   #206079

   Purpose

   Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

   Depositor

   Annette Dolphin

   Article

   Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

   Insert

   RAB11A (RAB11A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/Hygro-Mfn2-ActA

   Plasmid

   #191981

   Purpose

   Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

   Depositor

   Luca Scorrano

   Insert

   Mfn2-ActA
   

   Tags

   ActA

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EphA2 430-976 pcDNA3.1

   Plasmid

   #102738

   Purpose

   Expression of c terminal EphA2 in mammalian cells

   Depositor

   Jin Chen

   Article

   Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

   Insert

   EphA2 N terminal (430-976aa) (Epha2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) neo with Smad5 CDS

   Plasmid

   #61800

   Purpose

   pcDNA3.1(+) with full length mouse Smad5 CDS

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   Smad5 CDS (Smad5 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 AT1R DRY-AAY

    Plasmid

    #45759

    Depositor

    Robert Lefkowitz, Laszlo Hunyady

    Article

    Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

    Insert

    AT1R DRY-AAY (Agtr1a Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed amino acids 125-126 from DR to AA

    Promoter

    CMV

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 myc his Su(fu) (CT#768)

    Plasmid

    #13855

    Depositor

    Cliff Tabin

    Article

    Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

    Insert

    Su(fu) (SUFU Chicken)
    

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    full ORF

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-MS2-HA-HsRoquin1_510-1133_AE

    Plasmid

    #148613

    Purpose

    Mammalian Expression of HsRoquin1_510-1133

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1_510-1133 (RC3H1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-HA-FbxO11-Jeff-pcDNA3.1-

    Plasmid

    #52503

    Purpose

    expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Q491L (Jeff)

    Promoter

    CMV

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Flag-ZFP809(1-353)

    Plasmid

    #67788

    Purpose

    Mammalian expression of 1-353 amino acids of ZFP809 tagged with Flag

    Depositor

    Stephen Goff

    Article

    Wolf et al Nature. 2009 Apr 30;458(7242):1201-4. do

    Insert

    ZFP809 (Zfp809 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-Hyg-mEGFP-pp4640

    Plasmid

    #191846

    Purpose

    Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusion

    Depositor

    Bertrand Collet

    Article

    Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

    Insert

    mEGFP-pp4640
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    mEGFP fusion in Nterminal, some silent point muta…

    Promoter

    CMV

    Available Since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-2xFLAG-2xSTREP-CCND3

    Plasmid

    #172623

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cry2 mCherry PGK-1 in pcDNA3.1

    Plasmid

    #64214

    Purpose

    mCherry fused between Cry2 and PGK-1 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    PGK1 (PGK1 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. Mouse NCKX4-AS-long (pcDNA3.1+)

    Plasmid

    #75208

    Purpose

    Mammalian expression of SLC24A4

    Depositor

    Jonathan Lytton

    Article

    Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

    Insert

    SLC24A4 (Slc24a4 Mouse)
    

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-MS2-HA-HsMex3B_230-569_AD

    Plasmid

    #148480

    Purpose

    Mammalian Expression of HsMex3B_230-569

    Depositor

    Elisa Izaurralde

    Insert

    HsMex3B_230-569 (MEX3B Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/HisC 14-3-3 epsilon K49E

    Plasmid

    #11943

    Depositor

    Michael Yaffe

    Article

    Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

    Insert

    Ywhae (Ywhae Rat)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    K49E

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only