161,202 results

1. SUMO_MT3

   Plasmid

   #200303

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

   Insert

   metallothionein 3 (MT3 Human)
   

   Use

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBT9-ubiquitin

   Plasmid

   #196999

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

   Insert

   ubiquitin (UBC Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-V5-SBP-C1-HsUpf1-V161ER162E-siRNAres_Q

   Plasmid

   #147332

   Purpose

   Mammalian Expression of HsUpf1-V161ER162E

   Depositor

   Elisa Izaurralde

   Insert

   HsUpf1-V161ER162E (UPF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 non silent mutations A69S, E167G, K282R, A442G…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

   Plasmid

   #176310

   Purpose

   Expresses CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   CreN59_nMag_NLS_P2A_mCherry_IRES_puroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1alpha

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1delZn2delBRCT

   Plasmid

   #173939

   Purpose

   Bacterial expression of PARP1 lacking Zn2 and BRCT domains

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn2delBRCT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 97-213 and 374-520; V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrichoGate-2

   Plasmid

   #160702

   Purpose

   gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   gpdh
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Incorporation of BsaI restriction sites

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK556

   Plasmid

   #74277

   Purpose

   Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-1246 to serine, numerous silent m…

   Promoter

   T7

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MOS

   Plasmid

   #64120

   Purpose

   A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

   Depositor

   Linzhao Cheng

   Article

   Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

   Insert

   OCT4-2A-SOX2 (POU5F1, SOX2 Human)
   

   Use

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hRARα

   Plasmid

   #135397

   Purpose

   Human Retinoic Acid Receptor-alpha in pcDNA3.1(+) plasmid expression vector

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoic acid receptor-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1-spikeRBD::∆ACE2 SURF

    Plasmid

    #206957

    Purpose

    Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

    Depositor

    Xiaokun Shu

    Article

    Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

    Inserts

    sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
    ACE2 (ACE2 Human)
    

    Use

    Tags

    cSURF, mCherry, and nSURF

    Expression

    Mammalian

    Mutation

    17-615 and RBD (319-541)

    Promoter

    CMV

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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