161,202 results

1. pcDNA3.1_3xFlagEzh2 WT

   Plasmid

   #173717

   Purpose

   WT EZH2 expression plasmid with 3XFLAG tag

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUGW-VGLUT2-pHluorin

   Plasmid

   #207469

   Purpose

   Lentiviral expression of VGLUT2-pHluorin

   Depositor

   Robert Edwards

   Article

   Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

   Insert

   VGLUT2 (Slc17a6 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CAG-SPOTlight-U2GCR

   Plasmid

   #164819

   Purpose

   Reporter to measure ISR state-dependent protein synthesis

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   SPOTlight-U2GCR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+-Tornado-split-nLuc

   Plasmid

   #212612

   Purpose

   For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-split-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   C373T mutation in CVB3

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-X(4130)B1

   Plasmid

   #168018

   Purpose

   Expresses X(4130)B1-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   X(4130)B1-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-DIO-TVA-2A-oG

   Plasmid

   #178431

   Purpose

   Expresses TVA and optimized Rabies G (oG) protein in a FLEX cassette

   Depositor

   Edward Callaway

   Inserts

   TVA950
   Optimized G
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastbac1-Flag-FANCD2

   Plasmid

   #134904

   Purpose

   codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   FANCD2 (FANCD2 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   polyhedron

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPUR-hU6-sgRNA-Sirius-8XMS2

   Plasmid

   #121942

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL1777 (pEffector)

    Plasmid

    #160731

    Purpose

    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins and crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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