We narrowed to 2,365 results for: DAP

1. pGEMHE-MmCENPT-4NS-GFP

   Plasmid

   #237431

   Purpose

   Expresses Mus musculus CENP-T with 4 rat-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with 4 rat-specific residues in HFD (Cenpt Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   432 T to A; 463 I to T, 470 L to E, 485 Q to L

   Promoter

   T7

   Available Since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE-MmCENPT-RnHFD-4NS-GFP

   Plasmid

   #237432

   Purpose

   Expresses Mus musculus CENP-T with rat HFD and 4 mouse-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with Rattus norvegicus HFD and 4 mouse-specific residues in HFD (Cenpt Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   432 A to T; 463 T to I, 470 E to L, 485 L to Q in…

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-GGA3-VHS-GAT

   Plasmid

   #173719

   Purpose

   VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

   Depositor

   Juan Bonifacino

   Article

   Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

   Insert

   GGA3 (GGA3, GGA2, GGA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hsp70: mKate2 dynactin1-811

   Plasmid

   #105970

   Purpose

   heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   dynactin1-811 (DCTN1 Human)
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   contains only amino acids 1-811

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET3d-SF-DCTN1-TS

   Plasmid

   #169375

   Purpose

   E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

   Depositor

   Bernd Bukau

   Article

   Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

   Insert

   dynactin subunit 1 isoform 1 (DCTN1 Human)
   

   Use

   Tags

   6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

   Expression

   Bacterial

   Mutation

   Point mutations: A139T, I304T, Q322R, T516A, E577…

   Promoter

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GB_PVX (GB1464)

   Plasmid

   #160612

   Purpose

   PVX vector adapted to GB

   Depositor

   Diego Orzaez

   Insert

   PVX vector adapted to GB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTargetAmp

   Plasmid

   #228552

   Purpose

   Template for PCR amplification for gRNA cloning plasmid by in vivo cloning.

   Depositor

   Jeferson Gross

   Insert

   Ampicillin resistance
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBB modified

   Plasmid

   #22226

   Depositor

   David Baltimore

   Article

   Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpec5'

   Plasmid

   #228549

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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