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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,796 results for: CAN

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Showing: 1561 - 1580 of 13796 results

  8. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  9. pET-Sfp R4-4 mutant

    Plasmid
    #173491
    Purpose
    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.
    Depositor
    Jun Yin
    Article
    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d
    Insert
    Sfp-R44
    Tags
    His tag
    Expression
    Bacterial
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173491-plasmid-map-sequence-id-349047

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  16. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Article
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Available Since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  19. pCoofy44

    Plasmid
    #55185
    Purpose
    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…
    Expression
    Bacterial
    Promoter
    pPH (Polyhedrin promoter)
    Available Since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55185-plasmid-map-sequence-id-85559

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  20. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

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Showing: 1561 - 1580 of 13796 results