We narrowed to 5,032 results for: plasmid dna

1. LT2_14_GlyOx

   Plasmid

   #177711

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   pTRX2_5xUAS-ymYPET-tCYC1
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pELuc

   Plasmid

   #118056

   Purpose

   GBPart - ELuc Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   ELuc
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYZ172

   Plasmid

   #98409

   Purpose

   Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Inserts

   upstream homologous region to delete lys9 in genome by recombination
   downstream homologous region to delete lys9 in genome by recombination
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDrop

   Plasmid

   #184872

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pVAX-Help-SIINFEKL

   Plasmid

   #141350

   Purpose

   DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell epitopes

   Depositor

   Ton Schumacher

   Article

   Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

   Insert

   HELP-SIINFEKL-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR-Blunt_hNANOG

   Plasmid

   #112639

   Purpose

   Plasmid for PCR amplification of hNANOG DNA template for in vitro transcription

   Depositor

   Ganna Bilousova

   Article

   McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

   Insert

   hNANOG (NANOG Human)
   

   Use

   Pcr template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRJPaph-eBFP2

   Plasmid

   #67030

   Purpose

   Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Insert

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAGM4723-Del

   Plasmid

   #112207

   Purpose

   Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.

   Depositor

   Thomas Mock

   Article

   Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.755

   Type

   Empty backbone

   Use

   Golden gate assembly

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pANY2

   Plasmid

   #112196

   Purpose

   This plasmid is used for efficient cloning of DNA and expression of protein in E. coli.

   Depositor

   Yingfeng An

   Article

   Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

   Type

   Empty backbone

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC18-LucFF

    Plasmid

    #220496

    Purpose

    To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. aCE89

    Plasmid

    #204494

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    φC31 integrase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. aCE90

    Plasmid

    #204495

    Purpose

    TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    phiC31 recombinase
    WBeta recombinase
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    PhIF and TREtight

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX552-rScn2a-3xV5-Syn-miRFP670

    Plasmid

    #182566

    Purpose

    For rat Nav1.2 knockin with 3xV5 at the extracellular loop

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    rat Scn2a gRNA and 3xV5 donor DNA
    miRFP670
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Syn and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Zahra73-59

    Plasmid

    #23957

    Depositor

    Lawrence Cohen

    Insert

    VSP (tpte Zebrafish)
    

    Use

    Tags

    Cerulean and Citrulline

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Zahra76-77

    Plasmid

    #23959

    Depositor

    Lawrence Cohen

    Insert

    VSP (tpte Zebrafish)
    

    Use

    Tags

    Cerulean and Citrulline

    Expression

    Mammalian

    Mutation

    T156R

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOME0271P

    Plasmid

    #182610

    Purpose

    Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. Alt lncRNA

    Depositor

    Dirk Dittmer

    Insert

    HHV8 Alt lncRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOME0245M

    Plasmid

    #182607

    Purpose

    Reverse complement PAN-RNA pOME0244 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.

    Depositor

    Dirk Dittmer

    Insert

    Reverse compliment for PAN_RNA (pOME0244)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOME0243M

    Plasmid

    #182605

    Purpose

    Reverse complement antiRTA1kb pOME0242 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.

    Depositor

    Dirk Dittmer

    Insert

    Reverse compliment of antiRTA1kb (pOME0242)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmSKI8_AA

    Plasmid

    #148227

    Purpose

    Insect Expression of DmSKI8

    Depositor

    Elisa Izaurralde

    Insert

    DmSKI8 (CG3909 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TH mAb_BCN53.1.1D10_HC_Mouse IgG2a

    Plasmid

    #182896

    Purpose

    Mammalian expression of TH mouse mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-TH (human) heavy chain (TH Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TH mAb_BCN53.1.1D10_HC_Rat IgG2a

    Plasmid

    #182898

    Purpose

    Mammalian expression of TH rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-TH (human) light chain (TH Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TH mAb_BCN53.1.1D10_LC_Rat Ig Kappa

    Plasmid

    #182899

    Purpose

    Mammalian expression of TH rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-TH (human) light chain (TH Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TH mAb_BCN53.1.1D10_LC_Mouse Ig Kappa

    Plasmid

    #182897

    Purpose

    Mammalian expression of TH mouse mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-TH (human) light chain (TH Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CoV2-N-WT-Hu1

    Plasmid

    #177937

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHIE096 CMV Bxb1

    Plasmid

    #139252

    Purpose

    Encodes a Bxb1 recombinase for Landing Pad integration.

    Depositor

    Joshua Leonard

    Insert

    Bxb1 Recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPD1019 TRE3GV DsRedExpress2 in TU2

    Plasmid

    #139254

    Purpose

    TRE3GV promoter and DsRedExpress2 in TUPV2

    Depositor

    Joshua Leonard

    Insert

    DsRedExpress2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAMPK gamma1 H150R

    Plasmid

    #15997

    Depositor

    Morris Birnbaum

    Insert

    AMPK gamma1 (Prkag1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H150R

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pJM467 TRE3GV EYFP in TU3

    Plasmid

    #139255

    Purpose

    TRE3GV promoter and EYFP in TUPV3

    Depositor

    Joshua Leonard

    Insert

    EYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mouse Capping Protein Alpha2 EGFP

    Plasmid

    #229805

    Purpose

    Mamalian expression

    Depositor

    John Cooper

    Insert

    mouse capping protein Alpha2 EGFP fusion cDNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid

    #139257

    Purpose

    TRE3GV promoter and DsRedExpress2 in TUPV5

    Depositor

    Joshua Leonard

    Insert

    DsRedExpress2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CoV2-Spike-D614G

    Plasmid

    #177960

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid

    #177939

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G, N501Y

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pENTR_Hu_PRC1(WT)

    Plasmid

    #136349

    Purpose

    Human PCR1 cDNA in Gateway pENTR

    Depositor

    Guillermo de Cárcer

    Insert

    PRC1 (PRC1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR P4-P1R-ECFP

    Plasmid

    #48352

    Purpose

    Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR P2R-P3-SV40 polyA signal

    Plasmid

    #48627

    Purpose

    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    SV40 early polyadenylation signal cassette
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge