We narrowed to 2,231 results for: D10

1. pJT166_GalL_A3A-BE3

   Plasmid

   #145116

   Purpose

   Expressing base editor A3A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT139_GalL_nCDA1-VRERBE3

   Plasmid

   #145102

   Purpose

   Expressing base editor nCDA1-VRERBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-VRERBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT92_GalL_nCDA1Δ188-BE3

   Plasmid

   #145052

   Purpose

   Expresses nCDA1Δ188-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ188-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   pmCDA1(1-188AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT90_GalL_nCDA1Δ190-BE3

   Plasmid

   #145050

   Purpose

   Expresses nCDA1Δ190-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ190-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   pmCDA1(1-190AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT85_GalL_nCDA1Δ193-BE3

   Plasmid

   #145048

   Purpose

   Expresses nCDA1Δ193-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ193-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   pmCDA1(1-193AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT84_GalL_nCDA1Δ194-BE3

   Plasmid

   #145047

   Purpose

   Expresses nCDA1Δ194-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ194-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   pmCDA1(1-194AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT78_GalL_nCDA1Δ195-BE3

   Plasmid

   #145044

   Purpose

   Expresses nCDA1Δ195-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ195-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   pmCDA1(1-195AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Unc5b.z-Fc-His

   Plasmid

   #72179

   Purpose

   Expresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5b.z (Unc5b Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF-mTET1

   Plasmid

   #81052

   Purpose

   bacterial expression of murine TET1

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TET1 (Tet1 Mouse)
   

   Tags

   6HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ269E2

    Plasmid

    #164721

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-VPR_P2A_mCherry

    Plasmid

    #154193

    Purpose

    Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sp-dCas9-VPR_P2A_mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1as

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xFLAG-dCas9/pCMV-7.1

    Plasmid

    #47948

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A (catalyticall…

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpRY dCas9 KRAB

    Plasmid

    #188501

    Purpose

    Expresses FLAG tagged SpRY dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ABE8.20-m

    Plasmid

    #136300

    Purpose

    expresses ABE8.20-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-m
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

    Plasmid

    #134868

    Purpose

    Expresses EGFP-tagged lysosomal marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cbh_v5 AAV-ABE N-terminal

    Plasmid

    #137177

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-ABE N-terminal
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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