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We narrowed to 2,015 results for: D10

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Showing: 1561 - 1580 of 2015 results

  1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129048
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129048-plasmid-map-sequence-id-256223

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  2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129041
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129041-plasmid-map-sequence-id-256196

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  3. pLY152

    Plasmid
    #130950
    Purpose
    dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    PpspA-2G6 and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130950-plasmid-map-sequence-id-257362

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  4. CBP core-dCas9-p300 core

    Plasmid
    #179558
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179558-plasmid-map-sequence-id-354773

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  5. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid
    #208293
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208293-plasmid-map-sequence-id-410845

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  6. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

    Plasmid
    #192785
    Purpose
    Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192785-plasmid-map-sequence-id-379661

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  7. pLY164

    Plasmid
    #184140
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R3, PrhaB, and Ptet
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184140-plasmid-map-sequence-id-378329

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  8. pLY305

    Plasmid
    #184159
    Purpose
    Reporter circuit for hijacking mRNA of zraP as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    mutsfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    K140N, Mutations D10A and H840A on WT cas9. Synon…
    Promoter
    Pcon, PpspA-zraP-22, PrhaB, and Ptet
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184159-plasmid-map-sequence-id-376083

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  9. PF3D7_0422100-COMP-blac-flag-his

    Plasmid
    #110984
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    transmembrane emp24 domain-containing protein, putative (PF3D7_0422100 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    110984-plasmid-map-sequence-id-224371

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  10. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

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  12. M_5HN

    Plasmid
    #249755
    Purpose
    Minimal synthetic construct for assessing speckle localization containing a 5' splice site and an HNRNP motif-rich region
    Depositor
    Oded Regev
    Article
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_5HN
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available Since
    April 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    249755-plasmid-map-sequence-id-502717

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  16. M_B2

    Plasmid
    #249764
    Purpose
    Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5)
    Depositor
    Oded Regev
    Article
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_B2
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available Since
    April 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    249764-plasmid-map-sequence-id-508160

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  17. M_B3

    Plasmid
    #249765
    Purpose
    Minimal synthetic construct for assessing speckle localization, part of B trajectory series (starting with M_3HN5 and ending in M_3SR5)
    Depositor
    Oded Regev
    Article
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_B3
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    249765-plasmid-map-sequence-id-508127

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  18. M_A6

    Plasmid
    #249762
    Purpose
    Minimal synthetic construct for assessing speckle localization, part of A trajectory series (starting with M_3HN5 and ending in M_3SR5)
    Depositor
    Oded Regev
    Article
    Wen et al bioRxiv [Preprint]. 2025 May 28:2025.05.
    Insert
    M_A6
    Expression
    Mammalian
    Promoter
    TRE+CMV
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    249762-plasmid-map-sequence-id-508124

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Showing: 1561 - 1580 of 2015 results