We narrowed to 4,634 results for: HRE

1. His-ATG3-C264A

   Plasmid

   #202778

   Purpose

   ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop

   Depositor

   James Hurley

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   ATG3 (ATG3 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   C264A

   Promoter

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. His-ATG3-R265A

   Plasmid

   #202783

   Purpose

   ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

   Depositor

   James Hurley

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   ATG3 (ATG3 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   R265A

   Promoter

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. His-ATG3-H266A

   Plasmid

   #202784

   Purpose

   ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

   Depositor

   James Hurley

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   ATG3 (ATG3 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   H266A

   Promoter

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSC5-toxic3

   Plasmid

   #188625

   Purpose

   pSC5GGv2 with HindII Gene Insert

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Insert

   HindII
   

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   ACT1 intron insert

   Promoter

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPOLY_new

   Plasmid

   #179276

   Purpose

   Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   cellodextrin phosphorylase
   cellobiose phosphorylase
   sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
   

   Use

   Synthetic Biology

   Tags

   6xHIS and Strep-Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBICI_high ori

   Plasmid

   #179273

   Purpose

   Expresses CuCBP and BaSP in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDUBI

   Plasmid

   #179274

   Purpose

   Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   cellodextrin phosphorylase
   cellobiose phosphorylase
   C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
   

   Use

   Synthetic Biology

   Tags

   6xHIS and Strep-Tag II

   Expression

   Bacterial

   Mutation

   sucrose phosphorylase

   Promoter

   T7 lacO

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pInducer20 CARD8-HA PAM L368G

   Plasmid

   #169985

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, L368G

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pInducer20 CARD8-HA PAM R394E

   Plasmid

   #169987

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, R394E

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pInducer20 CARD8-HA PAM F405G

    Plasmid

    #169988

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, F405G

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pInducer20 CARD8-HA PAM F370G

    Plasmid

    #169986

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, F370G

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221 CARD8 FIIND F405G + S297A

    Plasmid

    #169974

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, F405G

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 CARD8 FIIND E274R + S297A

    Plasmid

    #169968

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, E274R

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 CARD8 FIIND R394E + S297A

    Plasmid

    #169972

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, R394E

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221 CARD8 PAM E274R

    Plasmid

    #169977

    Purpose

    pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    PAM, E274R

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

    Plasmid

    #169955

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, R394E

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

    Plasmid

    #169957

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, F405G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

    Plasmid

    #169951

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R, S297A

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tac(5A)-GFP

    Plasmid

    #162495

    Purpose

    Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The five threonine residues are mutated into alan…

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Tac(5A)

    Plasmid

    #162493

    Purpose

    Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The five threonine residues are mutated into alan…

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge