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LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZ

Plasmid

#192417

Purpose

To act as the 2-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1

Plasmid

#192414

Purpose

To act as the 2-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

Plasmid

#162815

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_M

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_M(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

Plasmid

#162816

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_9b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_9b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPA2

Plasmid

#19512

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pPROEX-HTc-Flag3-Nrf2

Plasmid

#21553

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Use

Tags

6xHis and Flag3

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Drp1 V2 S600D

Plasmid

#46345

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S600D

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Drp1 V2 S600A

Plasmid

#46344

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S600A

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Drp1 V2 S579D

Plasmid

#46343

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S579D

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Drp1 V2 S579A

Plasmid

#46342

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S579A

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS HSPA2

Plasmid

#19539

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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TB:2xP53_RE:MiniP

Plasmid

#133881

Purpose

2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

2 copies of the p53 DNA binding motif

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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OccludinMut_Flag

Plasmid

#86043

Purpose

Full length Occludin with mutation of PPPY domain at AA9-12 to PAAY

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

Insert

OccludinMut (OCLN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

PPPY domain at AA9-12 to PAAY; I52T and Q365P

Promoter

CMV

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00514

Plasmid

#39615

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG13951 (l(2)k10201 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00328

Plasmid

#34451

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG30420 (Atf-2 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
CoV2-Spike-D614G-Alpha

Plasmid

#177961

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )

Use

Tags

Expression

Mammalian

Mutation

del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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