We narrowed to 7,635 results for: PAC
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Plasmid#203809PurposePlasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).DepositorInsertABE_D3
UseCRISPRPromoterCMVAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPPac-hp
Plasmid#48123PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoterDepositorInsertsignal peptide of Pac
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACT5FVMF(f)
Plasmid#110549PurposeControl plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008DepositorInsertEGFP, FV LTRs
UseQpcr control for titering foamy virusPromoterpromoter: MLV LTRAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
HypaCas9
Plasmid#138557PurposeExpresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSDDepositorInsertHypaCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN692A, M694A, Q695A, H698APromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC575
Plasmid#127976PurposeAMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertble (bleomycin resistance marker)
UseFungal expressionExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-CM
Plasmid#203588PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.PAC
Plasmid#57828PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC1430
Plasmid#127981PurposeAMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
ExpressionBacterialPromoterlac UV5Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC025
Plasmid#227597PurposeLentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motifDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only