We narrowed to 128 results for: spycatcher

1. pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

   Plasmid

   #234546

   Purpose

   to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   ECS-dSC
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   mGFAP(ABC1D)

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCatcher003(K31TAG)-Talin rod-mScarletI

   Plasmid

   #210022

   Purpose

   Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI
   

   Use

   Tags

   C-terminal mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Talin head-SpyCatcher003(K31TAG)

   Plasmid

   #210023

   Purpose

   Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyCatcher003 with K31TAG (Amber stop codon) muta…

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

   Plasmid

   #200636

   Purpose

   Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

   Plasmid

   #235150

   Purpose

   Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   HaloTag linked to SpyCatcher3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

   Plasmid

   #210021

   Purpose

   Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Synthetic, Human)
   

   Use

   Tags

   MycTag, CTag

   Expression

   Mammalian

   Mutation

   Transferrin receptor transmembrane domain with Y2…

   Promoter

   CMV promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

   Plasmid

   #157674

   Purpose

   Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   SpyCatcher with ddFLN4, ELP, Histag and ybbR tag
   

   Use

   Tags

   ddFLN4, ELP, Histag and ybbR tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE80l B-CC-B

   Plasmid

   #196479

   Purpose

   Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC

   Depositor

   Fei Sun

   Insert

   B-CC-B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE80l BCB

   Plasmid

   #196472

   Purpose

   Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   BCB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQE80l B-CN-B

    Plasmid

    #196477

    Purpose

    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN

    Depositor

    Fei Sun

    Article

    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

    Insert

    B-CN-B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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