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pCEP4-FLAG-TAPBPR

Plasmid

#135500

Purpose

Mammalian expression of FLAG-tagged TAPBPR

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Use

Tags

Luminal FLAG epitope tag and Signal peptide from …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBPGUw-HACK-G4>QF2

Plasmid

#80277

Purpose

GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-TM-VC

Plasmid

#135499

Purpose

Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Use

Tags

Luminal/Extracellular FLAG epitope tag, Signal pe…

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pBP-P_tetR-PTet*

Plasmid

#204077

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

tetR repressor and PTet* tetracycline-inducible promoter

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-HRasCT

Plasmid

#176030

Purpose

Encoding membrane-targeted miRFP670

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

Membrane-targeted miRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBP-P_cymR-PCymRC

Plasmid

#204071

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

cymR repressor and PCymRC cumic acid-inducible promoter

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-ORF-xylE

Plasmid

#112780

Purpose

Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

xylE open reading frame

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pPBpuro-COX2-EBFP2

Plasmid

#184720

Purpose

Exogenous COX2

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

COX2-EBFP2 (PTGS2 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_152 [pBP1 ORI]

Plasmid

#210674

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Insert

pBP1 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TopBP1-mVen

Plasmid

#211415

Purpose

mammalian expression of human TopBP1 C-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-P_vanR-PVanCC

Plasmid

#204078

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

vanR repressor and PVanCC vanillic acid-inducible promoter

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBPK1520-SNCA-A53T-ng

Plasmid

#181739

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A53T (SNCA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR3c-R248C

Plasmid

#45712

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

R248C

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only
pBPK1520-HEK3-CTTins-ng

Plasmid

#180019

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U7

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA

Plasmid

#120162

Purpose

EGFP followed by the poly(A) site from human CENPB gene

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ErbB1(DEL1011)

Plasmid

#197366

Purpose

Encoding human ErbB1 mutant.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR mutant. (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

deleted amino acids 1011-1186

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-K660E

Plasmid

#45705

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K660E

Promoter

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR3c-S249C

Plasmid

#45713

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S249C

Promoter

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660N

Plasmid

#45707

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K660N

Promoter

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only