We narrowed to 1,879 results for: Ipp

1. pCIBN-EYFP-hLaminB1

   Plasmid

   #103800

   Purpose

   BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

   Plasmid

   #236231

   Purpose

   AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-mCherry-DMDEx23-eGFP

   Plasmid

   #211366

   Purpose

   Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

   Depositor

   Ulrich Lächelt

   Article

   Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

   Insert

   mCherry-DMDEx23-eGFP
   

   Expression

   Mammalian

   Mutation

   mCherry interrupted by mdx dystrophin exon 23 bet…

   Promoter

   CAG

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV hSyn INTRON mScarlet-CAAX2 WPRE

   Plasmid

   #236232

   Purpose

   AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mScarletI fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mScarletI

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGFP-mHP1gamma

   Plasmid

   #181902

   Purpose

   Fluorescently tagged HP1gamma

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1gamma (Cbx3 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMECP2-GFP

   Plasmid

   #181904

   Purpose

   Fluorescently tagged MECP2, GFP

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   MECP2 (Mecp2 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

   Plasmid

   #182425

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-mNeongreen-hGH (GH1 Human)
   VNp-LZ
   

   Tags

   Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCIBN-tagBFP-hLaminB1

   Plasmid

   #103799

   Purpose

   BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-hLaminB1 (CIB1, LMNB1 Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCIBN-EYFP-hTRF1

   Plasmid

   #103802

   Purpose

   BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-hTRF1 (CIB1, TERF1 Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-GFP-mHP1alpha

    Plasmid

    #181912

    Purpose

    Bacterial protein expression vector for GFP-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIBN-tagBFP-hTRF1

    Plasmid

    #103801

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF1 (CIB1, TERF1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIBN-LacI-tagRFP-T

    Plasmid

    #103810

    Purpose

    'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI-tagRFP-T (CIB1, lacI Mustard Weed, E. coli)
    

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. synapsin-hIRβ-mCherry

    Plasmid

    #228449

    Purpose

    Expressing a truncated, constitutively active human insulin receptor (IRβ) with mCherry in rat primary hippocampal neurons

    Depositor

    Olivier Thibault

    Article

    Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

    Insert

    human Insulin Receptor beta subunit (INSR Human)
    

    Use

    AAV

    Promoter

    neuron-specific synapsin promote

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV hSyn INTRON HaloTag-STIM1 WPRE

    Plasmid

    #236233

    Purpose

    AAV expression of mouse STIM1 internally tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-Stim1 (Stim1 Mouse)
    

    Use

    AAV

    Tags

    HaloTag

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV hSyn INTRON mEmerald-STIM1 WPRE

    Plasmid

    #236234

    Purpose

    AAV expression of mouse STIM1 internally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mEmerald-Stim1 (Stim1 Mouse)
    

    Use

    AAV

    Tags

    mEmerald

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMECP2-RFP

    Plasmid

    #181905

    Purpose

    Fluorescently tagged MECP2, RFP

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    MECP2 (Mecp2 Mouse)
    

    Tags

    RFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRE3G-ZSGreen1-mHP1alpha

    Plasmid

    #181913

    Purpose

    Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Tags

    ZSGreen1

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV x EF1a_EKAREN4-ires-puro

    Plasmid

    #167828

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV hSyn INTRON mCherry-Jph3 WPRE

    Plasmid

    #236237

    Purpose

    AAV expression of human Junctophilin3 N-terminally tagged with mCherry

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    Junctophilin 3 (JPH3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CMV-LAMP1-HaloTag

    Plasmid

    #164209

    Purpose

    Expresses HaloTag tagged LAMP1 under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EF1a-mEmerald-CAPRIN1

    Plasmid

    #164218

    Purpose

    Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF-1a

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPHR-mCherry-hNCL

    Plasmid

    #181886

    Purpose

    Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNCL (NCL Mustard Weed, Human)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV hSyn INTRON HaloTag-Jph3 WPRE

    Plasmid

    #236238

    Purpose

    AAV expression of human Junctophilin3 N-terminally tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    Junctophilin 3 (JPH3 Human)
    

    Use

    AAV

    Tags

    HaloTag

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PLEX-PGK-LAMP1-HaloTag

    Plasmid

    #164216

    Purpose

    pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Promoter

    PGK

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV x EF1a_EKAREN5-ires-blast

    Plasmid

    #167822

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV x EF1a_EKAREN5(TA)-ires-puro

    Plasmid

    #167823

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCIG-FGF13A

    Plasmid

    #220793

    Purpose

    To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    FGF13 (FGF13 Human)
    

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPHR-EYFP-hHP1β

    Plasmid

    #103829

    Purpose

    Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-hHP1β (CRY2, CBX1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PLEX-PGK-ANXA11-mCerulean

    Plasmid

    #164214

    Purpose

    pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Promoter

    PGK

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGFP-mHP1beta

    Plasmid

    #181901

    Purpose

    Fluorescently tagged HP1beta

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1beta (Cbx1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV x EF1a_EKAREN4-ires-blast

    Plasmid

    #167829

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLEX-PGK-LAMP1-EYFP

    Plasmid

    #164215

    Purpose

    pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    EYFP

    Promoter

    PGK

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCIBN-hTRF1-tagRFP-T

    Plasmid

    #103811

    Purpose

    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. miniTol2 x EF1a_EKAREN5 + PGK-blast

    Plasmid

    #167818

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

    Plasmid

    #167819

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

    Plasmid

    #167827

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

    Plasmid

    #167820

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pPHR-mCherry-hHP1β

    Plasmid

    #103830

    Purpose

    Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-hHP1β (CRY2, CBX1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. pLV x EF1a_EKAREN4(TA)-ires-puro

    Plasmid

    #167830

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

    Plasmid

    #167826

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. miniTol2 x EF1a_EKAREN4 + PGK-blast

    Plasmid

    #167825

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

    Plasmid

    #236241

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV1.2 (CACNA1C Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    human Synapsin 1

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

    Plasmid

    #236239

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV2.1 (CACNA1A Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid

    #236230

    Purpose

    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    

    Use

    AAV

    Tags

    mEmerald

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV hSyn INTRON HaloTag-Sec61b WPRE

    Plasmid

    #236229

    Purpose

    AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-Sec61b (SEC61B Synthetic, Human)
    

    Use

    AAV

    Tags

    HaloTag

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid

    #236245

    Purpose

    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    U6 and human Synapsin1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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