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Plasmid#77625Purpose3rd generation lentiviral gRNA plasmid targeting human SPEGDepositorInsertSPEG (SPEG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH35
Plasmid#175234PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33-EGFP
Plasmid#175792PurposeBacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantificationDepositorInsertNCK1 (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVTCRa_candidate1
Plasmid#164995PurposeExpression of candidate TCR #1 TCRalpha cDNADepositorInsertTRAV (TRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA3 gRNA (BRDN0001144943)
Plasmid#75942Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KA3DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA3 gRNA (BRDN0001148452)
Plasmid#75943Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KA3DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA3 gRNA (BRDN0001147852)
Plasmid#75944Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KA3DepositorInsertRPS6KA3 (RPS6KA3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-NGN2-BRN3A
Plasmid#165597PurposeDonor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.DepositorInsertsUseCRISPR and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1alpha, Endogenous CLYBL (splice acceptor…Available sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-2/3
Plasmid#91420PurposeProtein expression and purification of human SH3 domain construct NCK1-2/3DepositorInsertNCK1-2/3 (NCK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-3/3
Plasmid#91476PurposeProtein expression and purification of human SH3 domain construct NCK1-3/3DepositorInsertNCK1-3/3 (NCK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FRS2_IRS
Plasmid#109815PurposeProtein expression and purification of FRS2_IRSDepositorInsertFRS2_IRS (FRS2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only