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Showing: 141 - 160 of 230 results

  4. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  5. mr-GST full length

    Plasmid
    #112982
    Purpose
    For protein expression and purification of full-length Drosophila mr (APC2)
    Depositor
    Terry Orr-Weaver
    Insert
    mr (APC2) (mr Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. mr-GST N-term fusion

    Plasmid
    #112983
    Purpose
    For protein expression and purification of N-terminus of Drosophila mr (APC2)
    Depositor
    Terry Orr-Weaver
    Insert
    mr (APC2) (mr Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-term only
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112983-plasmid-map-sequence-id-217464

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  10. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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  11. pPMS-1791

    Plasmid
    #232286
    Purpose
    Protein Expression
    Depositor
    Jeremy Nathans
    Insert
    Apcdd1 (Apcdd1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232286-plasmid-map-sequence-id-461160

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  12. pPMS-2016

    Plasmid
    #232290
    Purpose
    Protein Expression
    Depositor
    Jeremy Nathans
    Insert
    Apcdd1 (Apcdd1 Mouse)
    Use
    Tags
    8X His-Avi-1D4
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232290-plasmid-map-sequence-id-461150

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  13. 4LG9

    Plasmid
    #60096
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBL1XR1:APC048-A06:C27585 (TBL1XR1 TBL1XR1:APC048-A06:C27585, Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Expression
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. 3JZN

    Plasmid
    #60123
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED:APC056-C06:C32050 (EED Human, EED:APC056-C06:C32050)
    Use
    Tags
    6xHis and GST
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 141 - 160 of 230 results