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Plasmid#229868PurposeEntry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)DepositorInsertreverse FRT3 (rFRT3)
UseTagsExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJQ200
Plasmid#78496PurposeBase vector for construction of series of suicide plasmids for gene replacement in ProteobacteriaDepositorTypeEmpty backboneUseSuicide vectorTagsExpressionMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHW1143
Plasmid#229866PurposeEntry vector with FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA in slot 4 (for SapTrap assembly)DepositorInsertFRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA
UseTagsExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYS2
Plasmid#117490PurposeGeneration of unmarked gene deletions in Mycobacterium smegmatisDepositorInsertgfp, hyg
UseDeletion generationTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet
Plasmid#85578PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC-CMV-Tet
Plasmid#85577PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHW1133
Plasmid#229869PurposeDestination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCEDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFC902
Plasmid#106904PurposeThe vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1DepositorInsertscas9
pyrG
U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSiM24-eGFP
Plasmid#67883PurposePlant Gene expression Vector pSIM24 containing eGFP reporterDepositorInserteGFP
UseTagsExpressionPlantMutationPromoterM24Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYS1
Plasmid#117488PurposeGeneration of unmarked gene deletions in Mycobacterium smegmatisDepositorInsertChe9c 60–61, sacB, aph
UseDeletion generationTagsExpressionMutationPromoterAcetamideAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBL6
Plasmid#50549PurposeExpresses mCherry under the PLtetO-1 promoterDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
phlFd-mLin
Plasmid#140793PurposeCMV-phlFo2-phlFR-2A-mCherry mammalian synthetic gene circuitDepositorInsertphlFd-mLinearizer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCph8
Plasmid#50552PurposeExpresses Cph8 under a modified PLtetO-1 promoterDepositorInsertCph8
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
phlFs-mLin
Plasmid#140792PurposeCMV-phlFo-phlFR-2A-mCherry mammalian synthetic gene circuitDepositorInsertphlFs-mLinearizer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-ACTB-C200-GFPfr1
Plasmid#169798PurposeTHe donor vector for the ACTB gene locus.DepositorInsertDonor sequence to ACTB gene for HR
UseHr donor vector for human actb gene.TagsExpressionMutationPartial sequence of GFP is inserted before the st…PromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet-YAP
Plasmid#85580PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liverDepositorInsertsCreER
YAP
UseCre/LoxTagsFlag (2x)ExpressionMammalianMutationPromoterApoE.HCR.hAAT and TREAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC-CMV-Tet-YAP
Plasmid#85579PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liverDepositorInsertsCreER
YAP
UseCre/LoxTagsFlag (2x)ExpressionMammalianMutationPromoterTREAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHW1387
Plasmid#229880PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTRDepositorInsertPmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
UseTagsGFP-C1ExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1392
Plasmid#229878PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTRDepositorInsertPsnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
UseTagsGFP-C1ExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only