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UBC-4xmNG-dMCP

Plasmid

#231093

Purpose

Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

4xmNG-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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minCMV-1xmNG-dMCP

Plasmid

#231091

Purpose

Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-tdMCP-YFP

Plasmid

#99151

Purpose

Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

Dimeric MS2-coat binding protein

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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minCMV-1xmNG-dPCP

Plasmid

#231092

Purpose

Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Rab1b

Plasmid

#226579

Purpose

Encodes Rab1b protein labeled with GFP

Depositor

Gia Voeltz

Article

Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

Insert

Rab1-B (Ras-related protein)

Tags

AcGFP1

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNY-352

Plasmid

#231049

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to guinea pig Fc

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNY-345

Plasmid

#231045

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to rabbit Fc

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNY-347

Plasmid

#231047

Purpose

Used for GFP staining

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to mouse Fc

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pPMS-2397

Plasmid

#231044

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to chicken Fc

Use

Affinity Reagent/ Antibody

Tags

8xHis

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNY-346

Plasmid

#231046

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to human Fc

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG

Plasmid

#46956

Purpose

Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pMVA

Plasmid

#121149

Purpose

This plamid was used for improving the production of DMAPP (IPP) in E.coli

Depositor

Minjuan Xu

Article

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )

Use

Synthetic Biology

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSB_syb25

Plasmid

#197992

Purpose

Expressed sybody 25 as a C-terminal myc-His-tagged construct

Depositor

Simon Newstead

Article

Parker et al Nat Struct Mol Biol. 2023 Jul 23. doi: 1

Insert

Sybody 25

Tags

Myc-His

Expression

Bacterial

Promoter

Arabinose

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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MZB207

Plasmid

#217843

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to AP3D1 amphipathic helix (2-15) fused to mGreenLantern

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

mGreenLantern

Tags

10x His, AP3D1 (2-15), and SUMO (SMT3)

Expression

Bacterial

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MS2_GFP

Plasmid

#61764

Purpose

Expresses MS2 coat protein fused to GFP

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

MS2 coat protein fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

Ubc promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNG-IRES-mScarlet-dPCP

Plasmid

#231090

Purpose

Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

mNG-IRES-mScarlet-dPCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNG-IRES-mScarlet-dMCP

Plasmid

#231089

Purpose

Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

mNG-IRES-mScarlet-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Sec31A

Plasmid

#226582

Purpose

Encodes Sec31A protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

Insert

Sec31A (Protein transport protein) (SEC31A Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec23A

Plasmid

#66609

Purpose

GFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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MCP-EGFP-PiggyBac

Plasmid

#190003

Purpose

Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-EGFP

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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