Skip to main content
Addgene
Showing: 141 - 160 of 367 results

  1. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PPhlF
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

    Enlarge

  2. pGS1112

    Plasmid
    #63784
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    Sapphire-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63784-plasmid-map-sequence-id-106474

    Enlarge

  3. pGS1122

    Plasmid
    #63790
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eSapphire-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63790-plasmid-map-sequence-id-106477

    Enlarge

  5. TFORF1125

    Plasmid
    #143093
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ENO1 (ENO1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143093-plasmid-map-sequence-id-399018

    Enlarge

  6. FRP2010

    Plasmid
    #159313
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized tSapphire under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    V2del, L231H
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159313-plasmid-map-sequence-id-311213

    Enlarge

  7. pAIP4_1UKF

    Plasmid
    #234214
    Purpose
    Heterologous protein expression of cysteine protease avirulence protein AvrPphB in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UKF
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234214-plasmid-map-sequence-id-464179

    Enlarge

  8. R777-E173 Hs.PIK3R2

    Plasmid
    #70457
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70457-plasmid-map-sequence-id-125906

    Enlarge

  9. R777-E174 Hs.PIK3R2-nostop

    Plasmid
    #70458
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70458-plasmid-map-sequence-id-125907

    Enlarge

  11. 1272 pBabe puroL Myr HA Akt3

    Plasmid
    #9019
    Depositor
    William Sellers
    Insert
    Akt3 (AKT3 Human)
    Use
    Retroviral
    Tags
    HA and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2005
    Availability
    Academic Institutions and Nonprofits only

  14. PIK3R2A

    Plasmid
    #42476
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIK3R2A (PIK3R2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. PIK3R2A

    Plasmid
    #42490
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIK3R2A (PIK3R2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. R777-E005 Hs.AKT3

    Plasmid
    #70289
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    70289-plasmid-map-sequence-id-125738

    Enlarge

  20. R777-E006 Hs.AKT3-nostop

    Plasmid
    #70290
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70290-plasmid-map-sequence-id-125739

    Enlarge

Showing: 141 - 160 of 367 results