We narrowed to 10,315 results for: plasmids 101

1. px459 EQR

   Plasmid

   #101732

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VQR

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCZGY3163

   Plasmid

   #101248

   Purpose

   GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   gfp11::rps-18 (rps-18 Nematode, Synthetic)
   

   Use

   Gateway

   Tags

   GFP11

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p458 VRER

   Plasmid

   #101728

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p330 VQR

   Plasmid

   #101730

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNSA-ARS-CRISPR-BlastR

   Plasmid

   #101009

   Purpose

   Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUDP003

   Plasmid

   #101164

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDD2002

    Plasmid

    #101198

    Purpose

    Name: pCBG68-LANApi. LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDD2005

    Plasmid

    #101219

    Purpose

    Name: pCBR-K14. K14 driving red luciferase reporter for CBR in pCBR backbone.

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    K14 driving red luciferase reporter for CBR in pCBR backbone.
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDD2024

    Plasmid

    #101368

    Purpose

    Name: pCBG68-RBPjkΔA. RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    RBPjk binding site A mutation in pCBG68-LANApi (p…

    Promoter

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDD2015

    Plasmid

    #101365

    Purpose

    Name: pCBR-K14ΔTATA. K14 TATA box mutation in pCBR-K14 (pDD2005).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    K14 TATA box mutation in pCBR-K14 (pDD2005)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    K14 TATA box mutation in pCBR-K14 (pDD2005)

    Promoter

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDD2028

    Plasmid

    #101371

    Purpose

    Name: pCBR-RBPjkΔA. RBPjk binding site A mutation in pCBR-K14 (pDD2005).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site A mutation in pCBR-K14 (pDD2005).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    RBPjk binding site A mutation in pCBR-K14 (pDD200…

    Promoter

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDD2026

    Plasmid

    #101369

    Purpose

    Name: pCBG68-LANApiΔTATA. LANApi TATA box mutation in pCBG68-LANApi (pDD2002).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    LANApi TATA box mutation in pCBG68-LANApi (pDD2002).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    LANApi TATA box mutation in pCBG68-LANApi (pDD200…

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDD2027

    Plasmid

    #101370

    Purpose

    Name: pCBR-LANApiΔTATA. LANApi TATA box mutation in pCBR-K14 (pDD2005).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    LANApi TATA box mutation in pCBR-K14 (pDD2005).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    LANApi TATA box mutation in pCBR-K14 (pDD2005).

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCSN061

    Plasmid

    #101725

    Purpose

    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDD2023

    Plasmid

    #101367

    Purpose

    Name: pCBR-RBPjkΔB. RBPjk binding site B mutation in pCBR-K14 (pDD2005).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site B mutation in pCBR-K14 (pDD2005).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    RBPjk binding site B mutation in pCBR-K14 (pDD200…

    Promoter

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDD2022

    Plasmid

    #101366

    Purpose

    Name: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    RBPjk binding site B mutation in pCBG68-LANApi (p…

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge