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Plasmid#1310DepositorInsertHsp104 Y257W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 257 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y466W
Plasmid#1311DepositorInsertHsp104 Y466W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 466 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y507W
Plasmid#1312DepositorInsertHsp104 Y507W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 507 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104b Y819W
Plasmid#1314DepositorInsertHsp104 Y819W (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationChanged Tyr 819 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMAL-EFSep
Plasmid#34627DepositorInsertEF-Sep (tufB E. coli)
UseTags6xHIS and MBPExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 Y819W
Plasmid#1307DepositorInsertHsp104 Y819W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 819 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET3a-AqAdk_Y52C
Plasmid#18090DepositorInsertadenylate kinase (kad A. aeolicus)
UseTagsExpressionBacterialMutationTyr 52 changed to CysPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 Y257W
Plasmid#1303DepositorInsertHsp104 Y257W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 257 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJC45BS104 Y507W
Plasmid#1305DepositorInsertHsp104 Y507W (HSP104 Budding Yeast)
UseTags10X His tagExpressionBacterialMutationChanged Tyr 507 to TrpPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTon1(acgfp1)
Plasmid#233648PurposeExpression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInserttarget sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onTagsExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-Rabaptin-5
Plasmid#199173PurposeExpresses GFP-Rabaptin-5 fusion in mammalian cellsDepositorInsertRabaptin-5 (RABEP1 Human)
UseTagsEGFPExpressionMammalianMutationContains Tyr 149 Ser substitution; insert starts …PromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 gamma (agf)
Plasmid#20354DepositorInsertpDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)
UseTagsFlagExpressionMammalianMutationReplace Thr 183 and Tyr 185 with Ala and Phe resp…PromoterAvailable sinceApril 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EGFAB(H306Y)
Plasmid#25771DepositorInsertLDL receptor EGFAB (LDLR Human)
UseTagsGSTExpressionBacterialMutationHis 306 to TyrPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
peT3a-AqAdk_MVGDH
Plasmid#18092DepositorInsertadenylate kinase (kad A. aeolicus)
UseTags6xHisExpressionBacterialMutationTyr 52 changed to Cys, Val 145 changed to Cys, …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 beta2 (agf)
Plasmid#20356DepositorInsertpDCNA3 Flag p38 beta(agf) (MAPK11 Human)
UseTagsFlagExpressionMammalianMutationReplace Thr 182 and Tyr 184 with Ala and Phe resp…PromoterAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKD-SepRS-EFSep
Plasmid#34623DepositorUseTagsExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtac and Ptac (from SepRS- operon like)Available sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR LAT-FFF/Zap70 iLID-Drop
Plasmid#171032PurposeLight-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.DepositorInsertLAT (LAT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB Y2105A
Plasmid#68726PurposePoint mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTORDepositorInsertFKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
UseTagsGST-taggedExpressionBacterialMutationFRB domain only aa2015-2114; Y2105APromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag p38 alpha (agf)
Plasmid#20816DepositorInsertpGSTag Flag p38 alpha (agf) (Mapk14 Mouse)
UseTagsFlagExpressionBacterialMutationReplace Thr 180 and Tyr 182 with Ala and Phe resp…PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2-EcYRS-EGFP*
Plasmid#191118PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafishDepositorInsertMutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only