We narrowed to 219 results for: ube2s

1. pSC-PreE2

   Plasmid

   #52264

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC-IntE2

   Plasmid

   #52265

   Purpose

   Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   Ubc9 GST fusion (UBE2I Human, Synthetic)
   

   Use

   Tags

   GST and GyrA-intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA4-RI1-332-MYC-His6

   Plasmid

   #185351

   Purpose

   Mammalian expression of Ribophorin I

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   Ribophorin I (RPN1 Human)
   

   Use

   Tags

   Myc, 6xHis

   Expression

   Mammalian

   Mutation

   aa 1-332 only

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLAG-OTUB1 T134R,C91S-pcDNA3.1

   Plasmid

   #118212

   Purpose

   Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 91 to serine and threonine 134 t…

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-FKBP-E2

   Plasmid

   #175243

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV3flag8HOIL-1L

   Plasmid

   #50016

   Purpose

   Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-His6-Ubc9

   Plasmid

   #133909

   Purpose

   Bacterial expression of His6-Ubc9

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3-AUP1-FLAG

   Plasmid

   #185336

   Purpose

   Mammalian expression of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-4T-3-TRC8 513-664

   Plasmid

   #185352

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   TRC8 (RNF139 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 513-664 only

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3-AUP1-FLAG 5E

    Plasmid

    #185341

    Purpose

    Assessing G2BR function in AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    Flag, 6xHis

    Expression

    Mammalian

    Mutation

    R382E/Q383E/K390E/R398E/R400E

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3-AUP1-FLAG DTM

    Plasmid

    #185337

    Purpose

    Assessing domain requirements of AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    deletion of aa 24-45, corresponding to the hairpi…

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-His6-Ubc9-K14R

    Plasmid

    #185759

    Purpose

    Bacterial expression of His6-Ubc9-K14R

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    K14R

    Promoter

    T7

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-hs-CDC34

    Plasmid

    #52731

    Purpose

    retroviral expression of human CDC34

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    CDC34 (CDC34 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-6P-1 AUP1 292-410

    Plasmid

    #185331

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-His6-Ubc9-C93A

    Plasmid

    #133910

    Purpose

    Bacterial expression of His6-Ubc9-C93A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 93 to Alanine

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-His6-Ubc9-C138A

    Plasmid

    #133911

    Purpose

    Bacterial expression of His6-Ubc9-C138A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 138 to Alanine

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCINeo-gp78 Mutant G2BR

    Plasmid

    #185355

    Purpose

    Assessing cellular requirement for G2BRgp78

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa 595-600 KRFLNK to AGAAGG

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6P-1 AUP1 61-410

    Plasmid

    #185334

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 61-610 only

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-AUP1 A397D

    Plasmid

    #185342

    Purpose

    Assessing G2BR function in AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    Flag, 6xHis

    Expression

    Mammalian

    Mutation

    A397D

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-G2BRAUP1

    Plasmid

    #185343

    Purpose

    Expression of soluble G2BR, amino acids 378-404 of human AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 aa 378-404 (G2BR) (AUP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 378-404 only

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge