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EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

Plasmid

#42185

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Gamma 3(F70A, F71A) (Gng3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

F70A, F71A

Promoter

cmv

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

Plasmid

#42188

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Gamma 3(K68A,K69A) (Gng3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K68A,K69A

Promoter

cmv

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pLV-IRF9

Plasmid

#71452

Purpose

Lentiviral expression of IRF9

Depositor

George Stark

Article

Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

Insert

IRF9 (IRF9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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nAChR alpha4 WT

Plasmid

#24271

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR alpha4 WT (Chrna4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
nAChR alpha4 YFP

Plasmid

#15245

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

alpha4-YFP-M (Chrna4 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
nAChR alpha4 CFP

Plasmid

#15244

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

alpha4-CFP-M (Chrna4 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF8-Middle-part

Plasmid

#64672

Purpose

Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Containing only middle part of RNF8 amino acids 1…

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pRC/RSV-rat Agt

Plasmid

#122104

Purpose

Expression of angiotensinogen

Depositor

John Chan

Article

Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

Insert

angiotensinogen (Agt Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

RSV promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-EGFP-CLASP2 340-1084 9xS/A

Plasmid

#24384

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP G protein Gamma 3(K68A,K69A,F70A,F71A) in pcDNA3.1

Plasmid

#42189

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Gamma 3(K68A,K69A,F70A,F71A) (Gng3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K68A,K69A,F70A,F71A

Promoter

CMV

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1

Plasmid

#42190

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

A73L,L74I,L75S

Promoter

CMV

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-CLASP2 512-650 9xS/A

Plasmid

#24410

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant that c…

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BQ.1.1

Plasmid

#194493

Purpose

expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (BQ.1.1) (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

R346T, K444T, N460K and c-terminal 18aa del on th…

Promoter

CMV

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3_SARS2_XBB

Plasmid

#194494

Purpose

expressing SARS-CoV-2 XBB spike protein for pseudovirus production

Depositor

David Nemazee

Article

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (XBB) (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

Promoter

CMV

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Use

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)Rigor-L-2xHA

Plasmid

#193718

Purpose

Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Esrrb

Plasmid

#71152

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Esrrb

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Estrogen related receptor, beta (Esrrb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

Plasmid

#193717

Purpose

Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Synthetic, Human)

Use

Tags

2xmNeongreen-FRB

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-hsKIF5B(1-560)Rigor-L-mVenus

Plasmid

#193720

Purpose

Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-hsKIF5B(1-353)GCN4-L-GFP

Plasmid

#193714

Purpose

Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Synthetic, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-353);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge