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pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

Plasmid

#216694

Purpose

Synchronize trafficking of GPI-AP from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
SBP-mApple-FIREtag-GPI

Tags

FIREtag, IL2 signal sequence, Streptavidin Bindin…

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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LBR EGFP

Plasmid

#128150

Purpose

Expression of lamin B receptor

Depositor

Jennifer Lippincott-Schwartz

Article

Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

Insert

lamin B receptor (LBR Human)

Expression

Mammalian

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 R6A

Plasmid

#198202

Purpose

FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

R6A

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE hSPC-eGFP-W

Plasmid

#36450

Depositor

Darrell Kotton

Article

Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

Inserts

human SP-C promoter
eGFP (eGFP )

Use

Lentiviral

Promoter

N/A and human SP-C promoter

Available Since

May 11, 2012

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Tags

Citrine

Expression

Mammalian

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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SIV_Rev

Plasmid

#236244

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Rev

Use

Lentiviral

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pB[vasa-Cas9 PUb-ZsGreen]

Plasmid

#169012

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster vasa

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-NCAPH

Plasmid

#31214

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NCAPH (NCAPH Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcw107-V5

Plasmid

#64649

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pp-mCAT1

Plasmid

#83948

Purpose

This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

mCAT1 (Slc7a1 )

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5-K130R

Plasmid

#13096

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5-K130R (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

K130R

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δβ-catenin-pcDNA3

Plasmid

#45772

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δβ-catenin (CDH1 Human)

Expression

Mammalian

Mutation

Ends at amino acid 844. Last 35 amino acids dele…

Promoter

CMV

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCW-OVOL2-HA

Plasmid

#184737

Purpose

Doxycycline-inducible lentiviral expression of OVOL2

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Insert

ovo like zinc finger 2 (OVOL2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet ON

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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mVenusN-Cavin3

Plasmid

#108889

Purpose

Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)

Tags

mVenus1-155

Expression

Mammalian

Mutation

altered codon usage

Available Since

March 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Expression

Mammalian

Promoter

CMV and T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Expression

Mammalian

Promoter

CMV and T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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mVenusC-Cavin3

Plasmid

#108890

Purpose

Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Available Since

March 23, 2019

Availability

Academic Institutions and Nonprofits only

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