We narrowed to 19,761 results for: INO
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Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD
Plasmid#141198PurposeExpresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell linesDepositorInsertMAP3K20 (MAP3K20 Human)
TagsStrep, HAExpressionMammalianMutationdeletion of last 27 amino acids and amino acids 6…PromoterCMVAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W
Plasmid#200465PurposeLentiviral vector expressing gRNA targeting human SMARCA2DepositorInsertSMARCA2(46) (SMARCA2 Human)
UseLentiviralAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-CCNE1
Plasmid#162553PurposeOverexpression of CCNE1DepositorAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)
Plasmid#217800PurposeVariant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promotersDepositorInsertPCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…PromoterCMV and T7Available SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65454PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPMutationH134R variantPromoterhSyn1Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5-ires-blast
Plasmid#167822PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mCerulean-Rab11
Plasmid#140574PurposeFor use in light-induced protein inactivation through the interaction with CRY2DepositorTagsmCeruleanExpressionMammalianPromoterCMV IE94Available SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry
Plasmid#172627PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1_D770A-mCherry
Plasmid#192260PurposeExpresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.DepositorAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only