We narrowed to 11,141 results for: hal

1. AP1muA-Halo-ALFA-polyA-G418 HR

   Plasmid

   #229681

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Halo, ALFA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPW3755 : HDR-HsIRE1a-10xGS-HaloTag

   Plasmid

   #185677

   Purpose

   HDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 homology arms (ERN1 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet28a-His6-Halo-TEV-Nrf2

   Plasmid

   #62455

   Purpose

   Halo-His6 tagged Nrf2 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Nrf2 (NFE2L2 Human)
   

   Use

   Tags

   His6-Halo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GluA1-scFv-Halo

   Plasmid

   #230055

   Purpose

   AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.Halo-Kif13B tail 442-1843

   Plasmid

   #162721

   Purpose

   Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   Kif 13B (Kif13b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT with deletion of the 441 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

   Plasmid

   #185678

   Purpose

   Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-HaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

   Plasmid

   #175529

   Purpose

   Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

   Plasmid

   #171437

   Purpose

   Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   c-Fos-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-SKII-3XHA-HALO-URA

   Plasmid

   #188932

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-URA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-Spe4-RfA-Halotag-stop

    Plasmid

    #186387

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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