We narrowed to 48,847 results for: ang

1. pCAG-LbCas12a-Plus-mCherry

   Plasmid

   #191230

   Purpose

   To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   LbCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R, R883K, R887A

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172668

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   ALDOB pegRNA and ALDOB_nick-sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172669

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   RNF2 pegRNA and RNF2_nick-sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCG150_double_phiC31_attB

   Plasmid

   #169693

   Purpose

   cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

   Depositor

   Shih-Peng Chan

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-SpCas9HF1-P2A-puro

   Plasmid

   #214357

   Purpose

   Expresses SpCas9HF1 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9HF1 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-5U6-sgRNAs-hsyn-EGFP

   Plasmid

   #112213

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   sgRNAs
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ICUE4

   Plasmid

   #181846

   Purpose

   Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   ICUE4 (RAPGEF3 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-RIα-ICUE4

   Plasmid

   #181848

   Purpose

   ICUE4 cAMP sensor tethered to the PKA RIα subunit.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
   

   Use

   Tags

   ECFP, PKA RIα, and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

    Plasmid

    #181853

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-KMS

    Plasmid

    #188038

    Purpose

    Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

    Insert

    KLF4, MYC, and SOX2 separated by 2A sequences (KLF4, MYC, SOX2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Use

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124428

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST1374-N-NLS-flag-linker-Cas9-D10A

    Plasmid

    #51130

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    D10A (nickase mutant)

    Promoter

    CMV

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124431

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Zebrafish (if expression level is too high in zeb…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid

    #196063

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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