We narrowed to 22,773 results for: crispr

1. pY036_ATP1A1_G5_Array

   Plasmid

   #86620

   Purpose

   Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBUN501

   Plasmid

   #50582

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   zCas9D10A
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Cas9D10A nickase, was derived from the zCas9 and …

   Promoter

   AtU6-26p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY036_ATP1A1_G3_Array

   Plasmid

   #86619

   Purpose

   Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

   Plasmid

   #120296

   Purpose

   AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   Rosa26 gRNA (SpCas9 scaffold)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

   Plasmid

   #113041

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

   Plasmid

   #113040

   Purpose

   AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

   Plasmid

   #113042

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRetro-UMI

   Plasmid

   #222686

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-dSa-VPR

    Plasmid

    #99651

    Purpose

    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

    Depositor

    George Church

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65-RTA activator

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcU6.3_gRNA_c1

    Plasmid

    #221381

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1

    Depositor

    James Briscoe, Tatjana Sauka-Spengler

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    G. gallus U6.3

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

    Plasmid

    #221449

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

    Plasmid

    #221450

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

    Plasmid

    #221451

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOliSpec5'

    Plasmid

    #228551

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOliSpec3'

    Plasmid

    #228550

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpec5'

    Plasmid

    #228549

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR6K-GFP-CASTIF

    Plasmid

    #199653

    Purpose

    R6K plasmid encoding a type I-F CAST but no CRISPR array

    Depositor

    IIya Finkelstein

    Insert

    CAST I-F systems
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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