We narrowed to 7,115 results for: trac

1. pTAG5

   Plasmid

   #176869

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTAG6E

   Plasmid

   #176870

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_6E
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC-1-CmYLCV-6

   Plasmid

   #176872

   Purpose

   MoClo Level 0 plasmid, contains CmYLCV-6 promoter

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   CmYLCV
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMOD_A4800EC

   Plasmid

   #176873

   Purpose

   Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOD_B4800EC

   Plasmid

   #176874

   Purpose

   Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTAG2

   Plasmid

   #176864

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTAG3

   Plasmid

   #176866

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTAG4

   Plasmid

   #176867

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCL21-decoder

   Plasmid

   #159448

   Purpose

   Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.

   Depositor

   Joerg Stelling

   Article

   Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

   Insert

   Pact1-lexA-ER-B112-phosphodegron-tCyc1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   act1

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCL33-decoder

    Plasmid

    #159451

    Purpose

    Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1mut-tetR-nls-malE-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1mut

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCL38-decoder

    Plasmid

    #159452

    Purpose

    Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1tet-LexA-ER-B112-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1tet

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCL12-decoder

    Plasmid

    #159447

    Purpose

    Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    PlexAtet-Citrine-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PlexAtet

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL7007

    Plasmid

    #136946

    Purpose

    Malachite green aptamer with stability hairpin, bacterial promoter

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

    Insert

    malachite green aptamer (MGA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LI F-G Tet

    Plasmid

    #104524

    Purpose

    LI resistance gene module

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Tetracycline resistance gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-kanMX6-P3nmt1-mCFP

    Plasmid

    #105136

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, Thomas Pollard

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P3nmt1-mCFP
    

    Use

    Tags

    3nmt1-mCFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    3nmt1

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-kanMX6-P3nmt1-mYFP

    Plasmid

    #105138

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, Thomas Pollard

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P3nmt1-mYFP
    

    Use

    Tags

    3nmt1-mYFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    3nmt1

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-hphMX6-3nmt1

    Plasmid

    #105162

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-3nmt1
    

    Use

    Tags

    3nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    3nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a - TryX

    Plasmid

    #89623

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Tryparedoxin
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-EPN-01*-(ΔLF)

    Plasmid

    #80045

    Purpose

    Expression of Vpr binding mutant EPN-01*

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*(L33R)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Vpr binding site LF45 in p6 mutated

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-EPN-01(ΔI)

    Plasmid

    #80048

    Purpose

    Expression of EPN-01 assembly mutant L33R

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(L33R)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L33R

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge