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pLenti-CAG-empty-ires-EGFP

Plasmid

#122953

Purpose

Lentiviral expression empty vector under CAG promoter with EGFP expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B WT

Plasmid

#123092

Purpose

Expresses 3xFLAG-LC3B wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lamin A-mRFP

Plasmid

#124268

Purpose

Wild-type Lamin A, C-terminally tagged with mRFP

Depositor

Eric Schirmer

Insert

LMNA (LMNA Human)

Tags

mRFP

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TyrRS-His

Plasmid

#124121

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tyrosyl-tRNA ligase (tyrS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mNeonGreen

Plasmid

#99135

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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