We narrowed to 875 results for: seph

1. FLAG-mCep290

   Plasmid

   #27381

   Depositor

   Joseph Gleeson

   Article

   Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

   Insert

   Cep290 (Cep290 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-short_BrokenHeart (no BC)

   Plasmid

   #86950

   Purpose

   AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   Brokenheart construct
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIG AKT3 E17K

   Plasmid

   #73050

   Purpose

   Human AKT3 E17K

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 E17K (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E17K

   Promoter

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-rBrokenheart

   Plasmid

   #203394

   Purpose

   AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   Brokenheart
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG mFoxg1-GFP wildtype

   Plasmid

   #73048

   Purpose

   C-terminal fusion of gfp Mouse wildtype Foxg1

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   mFoxg1 (Foxg1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-TEV-U1AF37MF77M

   Plasmid

   #168267

   Purpose

   Expresses human dmU1A with the F37M/F77M double mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   U1A RRM1 crystallization module (SNRPA Human)
   

   Use

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F37M and F77M

   Promoter

   T7 promotor

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIG AKT3 K177M

   Plasmid

   #73051

   Purpose

   Human AKT3 K177M

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 K177M (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K177M

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIG AKT3 S472A

   Plasmid

   #73052

   Purpose

   Human AKT3 S472A

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 S472A (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S472A

   Promoter

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIG AKT3 S472E

   Plasmid

   #73053

   Purpose

   Human AKT3 S472E

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 S472E (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid

    #133793

    Purpose

    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    CjeCas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V2-TGAA linker sgRNA architecture from Kim et al.…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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