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pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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IZ401: pMVP/Lenti/Neo-DEST

Plasmid

#121850

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK001: pMVP/SB/Hygro-DEST

Plasmid

#121867

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HE401: pMVP (L1-R5) shRNA

Plasmid

#121698

Purpose

pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JZ901: pMVP (L5-L4) EF1a promoter

Plasmid

#121727

Purpose

pMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG001: pMVP (L1-L4) TBG promoter

Plasmid

#121689

Purpose

pMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TBG promoter (Liver specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT504: pMVP/SB/eGFP-DEST

Plasmid

#121861

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT601: pMVP/SB/mCherry-DEST

Plasmid

#121862

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF601: pMVP (L1-R5) TBG promoter

Plasmid

#121695

Purpose

pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TBG promoter (Liver specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2_1-395_V

Plasmid

#147808

Purpose

Mammalian Expression of HsDCP2_1-395

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-395 (DCP2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu‐rnhB D101N

Plasmid

#108695

Purpose

For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

rnhB

Tags

GST

Expression

Bacterial

Mutation

D101N catalytic site mutation

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1 Insulator ObLiGaRe/EPB71

Plasmid

#90018

Purpose

Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

Depositor

Eric-Paul Bennett

Article

Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

Insert

MCS flanked by insulator and inverted ZFN binding sites

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK901: pMVP/SB/Zeo-DEST

Plasmid

#121868

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KJ101: pMVP (R4-R3) osTir1

Plasmid

#121742

Purpose

pMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

osTir1

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

9x myc epitope tag

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JN402: pMVP (R4-R3) Firefly Luciferase

Plasmid

#121741

Purpose

pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Firefly Luciferase

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG603: pMVP (L1-L4) TRE promoter

Plasmid

#121691

Purpose

pMVP L1-L4 entry plasmid, contains TRE promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG101: pMVP (L5-L4) TRE promoter

Plasmid

#121729

Purpose

pMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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AP1297‐1

Plasmid

#87247

Purpose

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Depositor

Geraldine Seydoux

Article

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Tags

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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LB801: pMVP/PB/eCFP-DEST

Plasmid

#121872

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KU701: pMVP (L5-L4) eGFP

Plasmid

#121707

Purpose

pMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO901: pMVP (L5-L4) BioID2

Plasmid

#121724

Purpose

pMVP L5-L4 entry plasmid, contains BioID2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term BioID2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

BioID2 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pUC 3GLA UAS HAi

Plasmid

#71763

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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IG703: pMVP (L1-R5) TRE promoter

Plasmid

#121697

Purpose

pMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh-139

Plasmid

#92287

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

Insert

dGW182 NED (gw Fly)

Tags

NHA

Expression

Insect

Mutation

dGW182 NED: aa 205-490

Promoter

Ac5

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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attB-GT-mNeonGreen-linker::pUC-GW-Kan_mod

Plasmid

#244895

Purpose

Minicircle producer plasmid

Depositor

Andrew Bassett

Article

Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

mNeonGreen

Use

Unspecified

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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SpG-IP

Plasmid

#242073

Purpose

Expresses Cas9-SpG in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpG

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_LEU3_SIR4

Plasmid

#190582

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LEU3 (LEU3 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_LYS14_SIR4

Plasmid

#190583

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LYS14 (LYS14 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_YRR1_SIR4

Plasmid

#190585

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

YRR1 (YRR1 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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