We narrowed to 10,495 results for: nar

1. pGEX4T3-GST-EGFP

   Plasmid

   #177136

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. JI401: Ad5 E1A/Hexon qRT-PCR standard

   Plasmid

   #131753

   Purpose

   Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Inserts

   Ad5 E1A gene fragment
   Ad5 Hexon gene fragment
   

   Use

   Standard for qrt-pcr

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP783_SpyCatcher-DNMT3A-CO

   Plasmid

   #211776

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavudin-DNMT3A
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP618_αGCN4-KRAB-CO

   Plasmid

   #211782

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-mCherry
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect mCherr…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. DEL-pHG165a

   Plasmid

   #90064

   Purpose

   LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   DEL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

   Plasmid

   #120555

   Purpose

   Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   Cas9, NES, ERT2 and GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

   Plasmid

   #199252

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity eSpCas9(1.1)-D10A nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-spCas9-N57

   Plasmid

   #169920

   Purpose

   Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-N57
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N57 is the PAI domain of SB100X

   Promoter

   CAG

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCBE4-gam

   Plasmid

   #124449

   Purpose

   Expresses dead Cas9 (dCas9)-CBE4-gam

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dCBE4-gam
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hnRNPA2_RRM2

    Plasmid

    #157866

    Purpose

    express His tagged hnRNPA2 RRM2

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid

    #211777

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Spy-EZH2
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. hnRNPA2_RRM1

    Plasmid

    #157865

    Purpose

    express His tagged hnRNPA2 RRM1

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBP-2_6.sensor

    Plasmid

    #162800

    Purpose

    Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    2_6_RNA-device
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPRv2 EGFP sgRNA1

    Plasmid

    #164932

    Purpose

    Expresses EGFP sgRNA1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP780_SpyCatcher-KRAB-CO

    Plasmid

    #211773

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-KRAB
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PVT1-luciferase

    Plasmid

    #113350

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry Codon 59 sgRNA

    Plasmid

    #109431

    Purpose

    MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry codon 59 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRESneo-Ago2

    Plasmid

    #215841

    Purpose

    Expression of AGO2

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

    Insert

    Human AGO2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKD077

    Plasmid

    #136473

    Purpose

    SSB-mTur2 IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-mTur2 (ssb E. coli)
    

    Use

    Tags

    mTurquoise2

    Expression

    Bacterial

    Mutation

    mTurquoise2 inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. ires-eGFP(+HAs) donor-1

    Plasmid

    #87865

    Purpose

    Express eGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PVT1-Tol2

    Plasmid

    #113381

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

22. pAAV-UbC-mEmerald Rab7a

    Plasmid

    #115238

    Purpose

    To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab7a fused to mEmerald (RAB7A Human)
    

    Use

    AAV

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    7 Amino Acids (21 Nucleotides) between mEmerald a…

    Promoter

    Human UbC

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAT-9218

    Plasmid

    #124225

    Purpose

    Bacterial SpCas9 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetR/TetA

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJ4M_hnRNPA2_FL_D290V

    Plasmid

    #139110

    Purpose

    Express FL hnRNPA2 D290V with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBABE MDER

    Plasmid

    #13494

    Depositor

    Stephen Tapscott

    Article

    Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

    Insert

    MyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    full length MyoD was fused to aa282-595 of Esr1. …

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-PU.1-ETS

    Plasmid

    #124652

    Purpose

    Chimeric ETS domain

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT7-EGFP-C1-HsXRN1-del1174-1649_AH

    Plasmid

    #148904

    Purpose

    Mammalian Expression of HsXRN1-del1174-1649

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsXRN1-del1174-1649 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LLP779_αGCN4-VP64-CO

    Plasmid

    #211772

    Purpose

    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-VP64
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect VP64 e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

    Plasmid

    #163719

    Purpose

    Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge